# US 10,118,945 B2 · Dereplication strain of Aspergillus nidulans

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- Number: US 10,118,945 B2
- Document ID: US10118945B2
- Status: Granted
- Kind: B2
- Application number: 15/338,696
- Filed: 2016-10-31
- Published: 2017-05-04
- Granted: 2018-11-06
- Earliest claimed: 2015-10-30
- Assignees: University of Kansas; University of Southern California
- Applicants: University of Kansas; The University of Southern California
- Inventors: Berl Oakley; Manmeet Ahuja; Ruth Entwistle; Christine Oakley; Yi-Ming Chiang; Clay Wang
- Web page: https://patentgrep.com/patent/US10118945B2

## CPC

- C07K 7/06: having 5 to 11 amino acids
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Modified fungal strains having deleted gene clusters are provided. The modified fungal strains include A. nidulans. The deleted gene clusters are selected from the group of gene clusters responsible for the biosynthesis of sterigmatocystin, emericellamides, asperfuranone, monodictyphenone, terrequinone, F9775A, F9775B, asperthecin, and both portions of the split cluster that makes austinol and dehydroaustinol. Methods for making compounds by culturing the fungus in a growth media and separating the compound from the fungus and/or separating the compound from the growth media are included, as are the compounds and compositions comprising them.

## Claims

### Claim 1

A modified strain of A. nidulans comprising a deletion of a combination of gene clusters comprising the sequence of SEQ ID NO:8.

### Claim 2 (depends on 1)

A method for making a compound comprising culturing the modified strain of A. nidulans of claim 1 in a growth media and separating the compound from the fungus and/or separating the compound from the growth media.