# US 10,118,177 B2 · Single column microplate system and carrier for analysis of biological samples

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,118,177 B2
- Document ID: US10118177B2
- Status: Granted
- Kind: B2
- Application number: 14/728,790
- Filed: 2015-06-02
- Published: 2015-12-03
- Granted: 2018-11-06
- Earliest claimed: 2014-06-02
- Assignees: Seahorse Bioscience
- Applicants: Seahorse Bioscience
- Inventors: Sarah Burroughs; Paul McGarr; Suzanne Armitstead; Jay S. Teich; Jason Dell'Arciprete
- Web page: https://patentgrep.com/patent/US10118177B2

## CPC

- B01L 3/5085: {for multiple samples, e.g. microtitration plates}
- B01L 3/50855: {using modular assemblies of strips or of individual wells}
- B01L 7/02: Water baths; Sand baths; Air baths
- B01L 9/523: {for multisample carriers, e.g. used for microtitration plates}
- B01L 2200/02: Adapting objects or devices to another
- B01L 2200/028: Modular arrangements
- B01L 2200/142: Preventing evaporation
- B01L 2300/0627: Sensor or part of a sensor is integrated
- B01L 2300/0829: Multi-well plates; Microtitration plates
- B01L 2300/0861: Configuration of multiple channels and/or chambers in a single devices
- B01L 2300/0893: having a very large number of wells, microfabricated wells
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

A multiwell microplate for holding liquid samples, and a method of use thereof. The multiwell microplate includes a frame defining a plurality of wells disposed in a single column, each well having an opening with a length l1. A moat is disposed about the plurality of wells. A plurality of walls traverses the moat, the walls defining a plurality of compartments, each compartment having a length l2 selected from a range of greater than l1 and less than 6l1. A multiwell microplate carrier includes a body defining a plurality of regions configured to hold a plurality of multiwell microplates in parallel, each multiwell microplate defining a single column of wells, and each of the regions defining a plurality of openings that are adapted to mate with the single columns of wells.

## Claims

### Claim 1

A multiwall microplate for holding liquid samples, the multiwell microplate comprising:
a frame defining
a plurality of wells disposed in a single column, each well, having an opening with a length l1;
a moat comprising a plurality of compartments and equalizer channels at each end of the multiwell microplate; and
a plurality of walls traversing the moat, the walls defining the plurality of compartments of the moat, each compartment having a length l2 selected from a range of greater than l1 and less than 6l1,
wherein the single column has at least two side walls, and at least two of the compartments are disposed on the at least two side walls of the single column of wells, and
wherein at least two of the compartments are in fluidic communication via the equalizer channel and the equalizer channels connect compartments disposed on opposing side wails of the column.

### Claim 2 (depends on 1)

The multiwell microplate of claim 1, wherein the well length l1 is selected from a range of 1 mm to 9 mm.

### Claim 3 (depends on 1)

The multiwell microplate of claim 1, wherein the plurality of wells comprises eight wells.

### Claim 4 (depends on 1)

The multiwell microplate of claim 1, wherein the moat comprises eight compartments.

### Claim 5 (depends on 1)

The multiwell plate of claim 1, wherein a depth of the two compartments in communication via the equalizer channel is less than a depth of compartments adjacent thereto.

### Claim 6 (depends on 1)

The multiwell microplate of claim 1, Wherein a depth of at least one compartment is less than a depth of one of the wells.

### Claim 7 (depends on 6)

The multiwell microplate of claim 6, wherein the depth of the at least one compartment is up to 50% of the depth of one of the wells.

### Claim 8 (depends on 1)

The multiwell microplate of claim 1, wherein a depth of a compartment proximate an end portion of the frame is less than a depth of a compartment disposed at a center portion of the frame.

### Claim 9 (depends on 1)

The multiwell microplate of claim 1, wherein all of the compartments have a substantially equal length.

### Claim 10 (depends on 1)

The multiwell microplate of claim 1, further comprising a filling tab defined on an end portion of the frame.

### Claim 11 (depends on 1)

The multiwell microplate of claim 1, wherein at least one well is opaque white.

### Claim 12 (depends on 1)

The multiwell microplate of claim 1, wherein at least one well is opaque black.

### Claim 13 (depends on 1)

The multiwell microplate of claim 1, the frame further comprising an indent on a lower edge thereof.