# US 10,117,919 B2 · Methods and materials for generating CD8+ T cells having the ability to recognize cancer cells expressing a HER2/neu polypeptide

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- Number: US 10,117,919 B2
- Document ID: US10117919B2
- Status: Granted
- Kind: B2
- Application number: 15/810,924
- Filed: 2017-11-13
- Published: 2018-03-08
- Granted: 2018-11-06
- Earliest claimed: 2012-02-17
- Assignees: Mayo Foundation for Medical Education and Research
- Applicants: Mayo Foundation for Medical Education and Research
- Inventors: Keith L. Knutson; Andrea M. Henle
- Web page: https://patentgrep.com/patent/US10117919B2

## CPC

- A61K 38/2013: {IL-2}
- A61K 38/193: {Colony stimulating factors [CSF]}
- A61K 38/208: {IL-12}
- A61K 39/001106: {Her-2/neu/ErbB2, Her-3/ErbB3 or Her 4/ErbB4}
- A61K 45/06: Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
- A61P 35/00: Antineoplastic agents
- A61P 37/04: Immunostimulants
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- C07K 14/71: for growth factors; for growth regulators
- A61K 2039/55566: {Emulsions, e.g. Freund's adjuvant, MF59}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

This document provides methods and materials for generating CD8− T cells having the ability to recognize cancer cells expressing a HER2/neu polypeptide. For example, methods and materials for using a polypeptide consisting of an SLAFLPESFD amino acid sequence in vivo or in vitro to generate CD8+ T cells having the ability to recognize and lyse cancer cells expressing a HER2/neu polypeptide are provided.

## Claims

### Claim 1

A composition comprising an immunogenically effective amount of a polypeptide and an adjuvant selected from the group consisting of a CpG oligonucleotide, monophosphoryl lipid A, and Montanide ISA-51, wherein the sequence of said polypeptide consists of the amino acid sequence set forth in SEQ ID NO:1.

### Claim 2 (depends on 1)

The composition of claim 1, wherein said adjuvant is said CpG oligonucleotide.

### Claim 3 (depends on 1)

The composition of claim 1, wherein said adjuvant is said monophosphoryl lipid A.

### Claim 4 (depends on 1)

The composition of claim 1, wherein said adjuvant is said Montanide ISA-51.