# US 10,117,910 B2 · Composition of natural vitamin C and fish scale collagen peptide and preparation method thereof

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- Number: US 10,117,910 B2
- Document ID: US10117910B2
- Status: Granted
- Kind: B2
- Application number: 15/312,616
- Filed: 2015-02-04
- Published: 2017-03-30
- Granted: 2018-11-06
- Earliest claimed: 2014-05-20
- Assignees: Hainan Meihetai Biotechnology Co., Ltd.
- Applicants: Hainan Meihetai Biotechnology Co., Ltd.
- Inventors: Gonghui Chen
- Web page: https://patentgrep.com/patent/US10117910B2

## CPC

- A61K 38/39: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
- A61K 31/375: Ascorbic acid, i.e. vitamin C; Salts thereof
- A61K 35/60: Fish, e.g. seahorses; Fish eggs
- A61K 36/185: Magnoliopsida (dicotyledons)
- A61K 36/736: Prunus, e.g. plum, cherry, peach, apricot or almond
- A61P 3/00: Drugs for disorders of the metabolism (of the blood or the extracellular fluid A61P7/00)
- A61P 3/02: Nutrients, e.g. vitamins, minerals
- A61P 19/02: for joint disorders, e.g. arthritis, arthrosis
- A61P 29/00: Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
- C12P 17/04: containing a five-membered hetero ring, e.g. griseofulvin {, vitamin C}
- C12P 21/005: {Glycopeptides, glycoproteins}
- C12P 21/06: produced by the hydrolysis of a peptide bond, e.g. hydrolysate products (preparing foodstuffs by protein hydrolysis A23J3/00)
- C12Y 304/00: Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- A61K 2236/00: Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12Y: ENZYMES

## Abstract

Disclosed is a composition of natural vitamin C and a fish scale collagen peptide, comprising the following components: natural vitamin C and a fish scale collagen peptide. Also disclosed is a method for preparing the above-mentioned composition.

## Claims

### Claim 1

A method of preparing the composition of natural vitamin C and a fish scale collagen peptide comprising following steps: (1) adding ultrapure water into a formula amount of the fish scale collagen peptide to prepare an aqueous solution; (2) adding a pH regulator into the aqueous solution of the fish scale collagen peptide to adjust a pH value to 5-7; and adding, under a negative pressure condition, a formula amount of the natural vitamin C to prepare a mixed solution of the natural vitamin C and the fish scale collagen peptide; (3) concentrating the mixed solution, obtained in the step (2), under a negative pressure to give a concentrate of ¼-½ of an original volume; and (4) cold drying, freeze drying or spray drying the concentrate under a negative pressure condition, to give the composition of natural vitamin C and the fish scale collagen peptide.

### Claim 2 (depends on 1)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 1, wherein in the step (1), mass of the ultrapure water is 5-7 times the total mass of the natural vitamin C and the fish scale collagen peptide.

### Claim 3 (depends on 1)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 1, wherein the pH regulator used in the step (2) for regulating a pH value is citric acid or malic acid.

### Claim 4 (depends on 1)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 1, wherein in the step (2) and the step (3), temperatures are controlled at 30-55° C. during processes of mixing and concentration.

### Claim 5 (depends on 1)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 1, wherein in the step (4), a temperature for spray drying is between 100 and 140° C.; a temperature during the process of cold drying is controlled at 40-52° C.; and a temperature during the process of freeze drying is controlled at −20 to −10° C.

### Claim 6 (depends on 1)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 1, wherein a range for the negative pressures (P) in the steps (2), (3) and (4) is: 0.01 MPa≤P<0.1 MPa.

### Claim 7 (depends on 1)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 1, wherein the natural vitamin C is extracted from Acerola cherry.

### Claim 8 (depends on 1)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 1, wherein a method of preparing the fish scale collagen peptide comprises following steps of: a. selecting fish scales as a raw material, and immersing the same in edible vinegar for softening under a negative pressure; b. cleaning, drying and sterilizing the softened fish scales, and adding water and an enzyme successively for enzymatic hydrolysis, wherein pressure is controlled as being negative, during a process of the enzymatic hydrolysis; c. adding activated carbon for decoloration and deodorization under a negative pressure, and filtering out insoluble substances, to give an enzymatic-hydrolyzed fish scale collagen peptide solution; and d. concentrating, under a negative pressure, the fish scale collagen peptide solution to 30-50% of the volume thereof, and obtaining the fish scale collagen peptide by falling film evaporation and drying.

### Claim 9 (depends on 8)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 8, wherein a range for the negative pressures (P) in the steps a, b, c and d is: 0.01 MPa≤P<0.1 MPa.

### Claim 10 (depends on 8)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 8, wherein in the step a, temperature of softening is 70-90° C., and duration of softening is 30-50 min.

### Claim 11 (depends on 8)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 8, wherein in the step b, a weight of water added is 5-20 times that of the fish scales, a weight of the enzyme added is 1%-4% of that of the fish scales, and the enzymatic hydrolysis is performed at a temperature controlled at 30-50° C., for 2-6 h.

### Claim 12 (depends on 8)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 8, wherein the enzyme is selected from the group consisting of neutral proteases and alkali proteases.

### Claim 13 (depends on 8)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 8, wherein in the step c, a proportion of the added activated carbon to a weight of the fish scales is 1%-4%, and the decoloration and deodorization are performed at a temperature controlled at 20-30° C., for 30-40 min.

### Claim 14 (depends on 8)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 8, wherein, in the step d, a temperature is controlled at 60-70° C. during a process of the concentrating, and a temperature is controlled at 100-140° C. during a process of the drying.

### Claim 15 (depends on 2)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 2, wherein a range for the negative pressures (P) in the steps (2), (3) and (4) is: 0.01 MPa≤P<0.1 MPa.

### Claim 16 (depends on 3)

The method of preparing the composition of natural vitamin C and a fish scale collagen peptide according to claim 3, wherein a range for the negative pressures (P) in the steps (2), (3) and (4) is: 0.01 MPa≤P<0.1 MPa.