# US 10,117,582 B2 · Medical hyperspectral imaging for evaluation of tissue and tumor > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,117,582 B2 - Document ID: US10117582B2 - Status: Granted - Kind: B2 - Application number: 15/709,381 - Filed: 2017-09-19 - Published: 2018-05-03 - Granted: 2018-11-06 - Earliest claimed: 2004-11-29 - Assignees: Hypermed Imaging, Inc. - Applicants: Hypermed Imaging, Inc. - Inventors: Svetlana V. Panasyuk; Jenny Freeman; Alexander Panasyuk - Web page: https://patentgrep.com/patent/US10117582B2 ## CPC - A61B 5/0091: {for mammography} - A61B 5/0059: {using light, e.g. diagnosis by transillumination, diascopy, fluorescence (photoacoustic A61B5/0093; optical measurement of heart rate A61B5/02416; optical measurement of blood flow A61B5/0261; optical measurement of analytes A61B5/1455)} - A61B 5/02007: {Evaluating blood vessel condition, e.g. elasticity, compliance} - A61B 5/415: {the glands, e.g. tonsils, adenoids or thymus} - A61B 5/416: {the spleen} - A61B 5/418: {lymph vessels, ducts or nodes} - A61B 5/4842: {Monitoring progression or stage of a disease} - A61B 5/742: {using visual displays (displays for heart-related electrical signals, e.g. ECG, A61B5/339)} - A61B 5/7425: {Displaying combinations of multiple images regardless of image source, e.g. displaying a reference anatomical image with a live image} - G01J 3/02: Details - G01J 3/0224: {using polarising or depolarising elements} - G01J 3/0264: {Electrical interface; User interface} - G01J 3/10: Arrangements of light sources specially adapted for spectrometry or colorimetry - G01J 3/2823: {Imaging spectrometer} - G01N 21/21: Polarisation-affecting properties (G01N21/19 takes precedence) - G01N 21/31: Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry {(G01N21/72 takes precedence)} - G01N 21/35: using infrared light (G01N21/39 takes precedence) - G01N 21/359: using near infrared light - G01N 2021/3137: {with selection of wavelengths after the sample} - G01N 2021/3155: {Measuring in two spectral ranges, e.g. UV and visible} - G01N 2021/317: {Special constructive features} - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - G01J: MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY (light sources F21, H01J, H01K, H05B; investigating properties of materials by optical means G01N) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Apparatus and methods for hyperspectral imaging analysis that assists in real and near-real time assessment of biological tissue condition, viability, and type, and monitoring the above overtime. Embodiments of the invention are particularly useful in surgery, clinical procedures, tissue assessment, diagnostic procedures, health monitoring, and medical evaluations, especially in the detection and treatment of cancer. ## Claims ### Claim 1 A method for displaying information about an in vivo tissue in a region of interest of a subject, the method comprising: co-registering corresponding pixels in each image in a plurality of images of the region of interest, each respective image in the plurality of images corresponding to radiation emitted at a particular spectral band in a plurality of different spectral bands; deriving a coefficient for oxyhemoglobin and a coefficient for deoxyhemoglobin from each set of co-registered pixels in the plurality of images; generating, based on the plurality of images, a two-dimensional index characterizing a particular characteristic of the in vivo tissue; and displaying the coefficient for oxyhemoglobin and the coefficient for deoxyhemoglobin from at least one set of co-registered pixels in the plurality of images independently with the two-dimensional index. ### Claim 2 (depends on 1) The method of claim 1, wherein at least one respective spectral band in the plurality of spectral bands corresponds to radiation emitted in the visible spectrum. ### Claim 3 (depends on 1) The method of claim 1, wherein at least one respective spectral band in the plurality of spectral bands corresponds to radiation emitted in the near-infrared spectrum. ### Claim 4 (depends on 1) The method of claim 1, wherein the coefficient for oxyhemoglobin and the coefficient for deoxyhemoglobin from at least one set of co-registered pixels in the plurality of images and the two-dimensional index are displayed on a video display. ### Claim 5 (depends on 1) The method of claim 1, wherein each respective spectral band in the plurality of spectral band has a bandwidth of less than 20 nm. ### Claim 6 (depends on 1) The method of claim 1, wherein each spectral band in the plurality of spectral bands has a bandwidth that is between 10 nm and 40 nm. ### Claim 7 (depends on 1) The method of claim 1, wherein each spectral band in the plurality of spectral bands has a bandwidth that is between 10 nm and 15 nm. ### Claim 8 (depends on 1) The method of claim 1, wherein the two-dimensional index includes information about at least one of a presence of a tumor, a presence of a residual tumor at a margin of a surgical excision bed, and a progression of a tumor. ### Claim 9 (depends on 8) The method of claim 8, wherein the information about the progression of the tumor includes at least one of a tumor stage grading and a microvascular change in a vascular tissue. ### Claim 10 (depends on 1) The method of claim 1, wherein the region of interest of the subject is a portion of the skin of the subject. ### Claim 11 (depends on 1) The method of claim 1, further comprising a step of recording the plurality of images prior to the co-registering step. ### Claim 12 (depends on 11) The method of claim 11, wherein the recording of images is performed using a multispectral or hyperspectral medical imaging system comprising: a plurality of lights; a first stage optic configured to receive radiation projected, from one or more respective lights in the plurality of lights, off of the in vivo tissue; one or more bandpass filters in optical communication with the first stage optic; an imaging sensor for recording an image of the radiation projected off of the in vivo tissue; a diagnostic protocol module adapted to detect a particular characteristic of the in-vivo tissue; and a diagnostic processor configured to: switch one or more respective lights in the plurality of lights on and off based on the diagnostic protocol module, and instruct the imaging sensor to record a plurality of images of the region of interest based on a diagnostic protocol module.