# US 10,117,432 B2 · Isoflavonoid compounds and use thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,117,432 B2 - Document ID: US10117432B2 - Status: Granted - Kind: B2 - Application number: 11/919,886 - Filed: 2005-03-18 - Published: 2009-12-10 - Granted: 2018-11-06 - Earliest claimed: 2004-03-18 - Assignees: Novozymes Bioag A/S - Applicants: John McIver; Chunquan Chen; Birgit Schultz; Hannah McIver - Inventors: John McIver; Chunquan Chen; Birgit Schultz; Hannah McIver - Web page: https://patentgrep.com/patent/US10117432B2 ## CPC - A01N 43/16: with oxygen as the ring hetero atom - A01N 25/00: Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application {, e.g. seed treatment or sequential application}; Substances for reducing the noxious effect of the active ingredients to organisms other than pests - A01N 43/32: six-membered rings - Y02A 40/10: in agriculture - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract The use of a one or more Isoflavonoid compound Signals which may be with an agriculturally acceptable carrier, applied prior to planting, up to 365 days or more, either directly to the seed or transplant of a non-legume crop or a legume crop, or applied to the soil that will be planted either to a non-legume crop or a legume crop, for the purpose of increasing yield and/or improving seed germination and/or improving carrier seed emergence and/or improving modulation and/or increasing crop stand density and/or improving plant vigour and/or improving plant growth, and/or increasing biomass, and/or earlier fruiting, all including in circumstances of seeding and plant transplanting. ## Claims ### Claim 1 A method of improving plant growth, comprising: applying, to a non-leguminous seed, a composition that comprises at least one isoflavonoid and that is devoid of nitrogen-fixing bacteria and nod factors, wherein, upon planting said seed in a growth medium that comprises one or more nitrogen-fixing bacteria, application of said composition results in improved germination of said seed and/or earlier emergence, increased yield, increased stand density, improved vigor, improved growth, increased biomass and/or earlier fruiting of a plant that germinates from said seed, as compared to an untreated control seed. ### Claim 2 (depends on 1) The method of claim 1, wherein said composition is applied to said seed one month before said seed is planted. ### Claim 3 (depends on 1) The method of claim 1, wherein said composition is applied to said seed 60 days before said seed is planted. ### Claim 4 (depends on 1) The method of claim 1, wherein said composition is applied to said seed 365 days or more before said seed is planted. ### Claim 5 (depends on 1) The method of claim 1, wherein said composition further comprises one or more herbicides, fungicides, insecticides, fertilizers and/or growth promoters. ### Claim 6 (depends on 1) The method of claim 1, wherein said at least one isoflavonoid comprises genistein. ### Claim 7 (depends on 1) The method of claim 1, wherein said at least one isoflavonoid comprises daidzein. ### Claim 8 (depends on 1) The method of claim 1, wherein said at least one isoflavonoid comprises genistein and daidzein. ### Claim 9 (depends on 1) The method of claim 1, wherein said plant is grown under growth-limiting conditions. ### Claim 10 (depends on 1) The method of claim 1, wherein said plant is grown under pH stress conditions. ### Claim 11 (depends on 1) The method of claim 1, wherein said plant is grown under water stress conditions. ### Claim 12 (depends on 1) The method of claim 1, wherein said plant is grown under temperature stress conditions. ### Claim 13 (depends on 1) The method of claim 1, wherein said seed is a corn seed. ### Claim 14 (depends on 1) The method of claim 1, wherein said seed is a tomato seed. ### Claim 15 (depends on 1) The method of claim 1, wherein said seed is a wheat seed. ### Claim 16 (depends on 1) The method of claim 1, wherein said method further comprises applying said one or more nitrogen-fixing bacteria to said growth medium. ### Claim 17 (depends on 16) The method of claim 16, wherein said one or more nitrogen-fixing bacteria are applied to said growth medium in an amount ranging from 500,000 to 5,000,000 cells per gram of growth medium. ### Claim 18 (depends on 16) The method of claim 16, wherein said one or more nitrogen-fixing bacteria are applied to said growth medium in an amount ranging from 10,000 to 1,000,000 cells per gram of growth medium. ### Claim 19 (depends on 16) The method of claim 16, wherein said one or more nitrogen-fixing bacteria are applied to said growth medium in an amount ranging from 103 to 105 cells per gram of growth medium. ### Claim 20 (depends on 16) The method of claim 16, wherein said one or more nitrogen-fixing bacteria are applied to said growth medium at concentration of about 100,000 cells per gram of growth medium. ### Claim 21 (depends on 16) The method of claim 16, wherein said one or more nitrogen-fixing bacteria comprises a strain of Bradyrhizobium japonicum. ### Claim 22 (depends on 1) The method of claim 1, wherein, upon planting said seed in a growth medium that comprises one or more nitrogen-fixing bacteria, application of said composition results in improved germination of said seed, as compared to an untreated control seed. ### Claim 23 (depends on 1) The method of claim 1, wherein, upon planting said seed in a growth medium that comprises one or more nitrogen-fixing bacteria, application of said composition results in earlier emergence, increased yield, increased stand density, improved vigor, improved growth, increased biomass and/or earlier fruiting of a plant that germinates from said seed, as compared to a plant that germinates from an untreated control seed. ### Claim 24 (depends on 1) The method of claim 1, wherein, upon planting said seed in a growth medium that comprises one or more nitrogen-fixing bacteria, application of said composition results in increased yield of a plant that germinates from said seed, as compared to a plant that germinates from an untreated control seed. ### Claim 25 (depends on 1) The method of claim 1, wherein, upon planting said seed in a growth medium that comprises one or more nitrogen-fixing bacteria, application of said composition results in improved growth of a plant that germinates from said seed, as compared to a plant that germinates from an untreated control seed. ### Claim 26 (depends on 1) A seed treated according to the method of claim 1, wherein said seed exhibits improved germination when planted in a growth medium that comprises one or more nitrogen-fixing bacteria, as compared to an untreated control seed. ### Claim 27 (depends on 1) A seed treated according to the method of claim 1, wherein a plant that germinates from said seed exhibits earlier emergence, increased yield, increased stand density, improved vigor, improved growth, increased biomass and/or earlier fruiting when planted in a growth medium that comprises one or more nitrogen-fixing bacteria, as compared to a plant that germinates from an untreated control seed.