# US 10,117,428 B2 · Compositions and methods for preserving donated blood

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- Number: US 10,117,428 B2
- Document ID: US10117428B2
- Status: Granted
- Kind: B2
- Application number: 14/905,341
- Filed: 2014-07-17
- Published: 2016-06-09
- Granted: 2018-11-06
- Earliest claimed: 2013-07-17
- Assignees: RythRx Therapeutics, LLC
- Applicants: Rythrx Therapeutics, LLC
- Inventors: Narayanan Surendran
- Web page: https://patentgrep.com/patent/US10117428B2

## CPC

- A01N 1/126: Physiologically active agents, e.g. antioxidants or nutrients
- A01N 1/122: Preservation or perfusion media
- A61K 31/519: ortho- or peri-condensed with heterocyclic rings
- A61K 31/7064: containing condensed or non-condensed pyrimidines
- C12N 5/0641: {Erythrocytes}
- C12N 5/0644: {Platelets; Megakaryocytes}
- C12N 2501/999: Small molecules not provided for elsewhere
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Blood and platelet storage and rejuvenating compositions that include triciribine, triciribine metabolites, triciribine analogs, and mixtures of the same are disclosed. Such compositions can be useful in methods for treating (e.g., storing and rejuvenating) red blood cells and platelets.

## Claims

### Claim 1

A method for preserving donated blood for subsequent transfusion into a patient, comprising
contacting donated blood with triciribine.

### Claim 2 (depends on 1)

The method of claim 1, further contacting the donated blood with at least one of an anticoagulant, buffer, and an additive solution.

### Claim 3 (depends on 1)

The method of claim 1, wherein the triciribine has one or more deuterium substitutions.

### Claim 4 (depends on 1)

The method of claim 1, further comprising refrigerating the donated blood.

### Claim 5

A method for preserving donated platelets for subsequent transfusion into a patient, comprising
contacting donated platelets triciribine.

### Claim 6 (depends on 5)

The method of claim 5, further comprising contacting the donated platelets with at least one of an anticoagulant, buffer, and an additive solution.

### Claim 7 (depends on 5)

The method of claim 5, wherein the triciribine has one or more deuterium substitutions.

### Claim 8 (depends on 5)

The method of claim 5, further comprising refrigerating the donated platelets.

### Claim 9 (depends on 3)

The method of claim 3, wherein a level of 2,3-diphosphoglycerate in the donated red blood cells is maintained during storage.

### Claim 10

A method of preserving donated blood for subsequent transfusion into a patient, comprising contacting donated red blood cells with triciribine so that formation of microparticles is reduced by at least 40% after 12 days compared to untreated red blood cells.

### Claim 11 (depends on 10)

The method of claim 10, comprising contacting the donated red blood cells with at least one of an anticoagulant, a buffer, and an additive solution.

### Claim 12 (depends on 10)

The method of claim 10, wherein the triciribine has one or more deuterium substitutions.

### Claim 13 (depends on 10)

The method of claim 10, further comprising refrigerating the donated blood.

### Claim 14 (depends on 2)

The method of claim 2, further comprising refrigerating the donated blood.

### Claim 15 (depends on 3)

The method of claim 3, further comprising refrigerating the donated blood.

### Claim 16 (depends on 6)

The method of claim 6, further comprising refrigerating the donated platelets.

### Claim 17 (depends on 7)

The method of claim 7, further comprising refrigerating the donated platelets.

### Claim 18 (depends on 11)

The method of claim 11, further comprising refrigerating the donated blood.

### Claim 19 (depends on 12)

The method of claim 12, further comprising refrigerating the donated blood.