# US 10,117,390 B2 · Use of genic male sterility gene and mutation thereof in hybridization

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- Number: US 10,117,390 B2
- Document ID: US10117390B2
- Status: Granted
- Kind: B2
- Application number: 14/917,477
- Filed: 2014-09-15
- Published: 2016-09-08
- Granted: 2018-11-06
- Earliest claimed: 2013-09-16
- Assignees: Xingwang Investment Co., Ltd.
- Applicants: Xingwang Investment Co., Ltd
- Inventors: Junli Zhou; Ying Wang
- Web page: https://patentgrep.com/patent/US10117390B2

## CPC

- C12N 15/8289: {Male sterility}
- A01H 1/023: {Male sterility}
- A01H 5/00: Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H 5/10: Seeds
- A01H 6/4684: {Zea mays [maize]}
- C07K 14/415: from plants
- C12N 15/63: Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N 15/82: for plant cells {, e.g. plant artificial chromosomes (PACs)}
- C12N 15/8216: {Methods for controlling, regulating or enhancing expression of transgenes in plant cells}
- C12N 15/8231: {Male-specific, e.g. anther, tapetum, pollen}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention belongs to the field of biotechnology, in particular to a hybrid breeding method for maize, which comprises sterile line reproduction and hybrid seed production, and more particularly to plant FL1 gene or alleles thereof, as well as mutant plants produced by the variation of the gene.

## Claims

### Claim 1

An isolated nucleotide sequence comprising the nucleotide sequence of SEQ ID NO:2 or the full length complementary sequence of SEQ ID NO:2.

### Claim 2 (depends on 1)

The isolated nucleotide sequence of claim 1, consisting of SEQ ID NO:2.

### Claim 3 (depends on 1)

An expression cassette, comprising the nucleotide sequence of claim 1 operably linked to a promoter sequence.

### Claim 4 (depends on 3)

The expression cassette of claim 3, wherein the promoter sequence comprises SEQ ID NO:4 or SEQ ID NO:5.

### Claim 5 (depends on 3)

An expression vector, comprising the expression cassette of claim 3.

### Claim 6 (depends on 5)

An engineered bacterium, wherein the engineered bacterium contains the expression vector of claim 5.

### Claim 7 (depends on 1)

An expression vector, comprising the nucleotide sequence SEQ ID NO:4 or SEQ ID NO:5, operably linked to the nucleotide sequence of claim 1.

### Claim 8 (depends on 7)

An engineered bacterium, wherein the engineered bacterium contains the expression vector of claim 7.

### Claim 9 (depends on 7)

A method for expressing a target nucleotide sequence in a plant, the method comprises: introducing the expression vector of claim 7 into the plant, wherein the target nucleotide sequence is expressed.

### Claim 10 (depends on 9)

The method of claim 9, wherein the plant is a monocotyledon.

### Claim 11 (depends on 10)

The method of claim 10, wherein the monocotyledon is Gramineae.

### Claim 12 (depends on 11)

The method of claim 11, wherein the Gramineae is rice, maize or sorghum.