Biomarkers for pneumonia and acute decompensated heart failure
AssigneeUpstream Medical Technologies Limited
InventorsChristopher Joseph Pemberton, Arthur Mark Richards, Matthew Simon Byers
Life of this patent
Estimating the term…
Abstract
Ghrelin signal peptide fragment assays and kits useful in the diagnosis, prognosis, risk stratification, assessing, staging, monitoring, categorizing and determination of further diagnoses and treatment regimens in subjects with various disorders, diseases and conditions including, pneumonia, heart failure, or pneumonia and heart failure or suspected pneumonia, heart failure, or pneumonia and heart failure, and methods for monitoring treatment.
Claims24
A method of monitoring a subject having both pneumonia and acute decompensated heart failure, comprising
- (a) performing an immunoassay to determine a level of a ghrelin signal peptide (GHRsp) fragment by contacting a body fluid sample obtained from the subject with an antibody or antigen-binding antibody fragment thereof that binds a ghrelin signal peptide fragment that is (a) GHRsp (1-9) (SEQ ID NO:3);
- or (b) GHRsp (1-10) (SEQ ID NO:4) and detecting ghrelin signal peptide fragment bound to the antibody or antigen-binding antibody fragment thereof;
- (b) comparing the level of the ghrelin signal peptide fragment determined by the immunoassay with a level of the ghrelin signal peptide fragment in a reference body fluid sample obtained from the same subject at an earlier time, which reference body fluid sample contained a level of ghrelin signal peptide fragment that was above a threshold level of the ghrelin signal peptide fragment that distinguishes a population of subjects having both pneumonia and acute decompensated heart failure from a population of subjects not having both pneumonia and acute decompensated heart failure, wherein a level of ghrelin signal peptide fragment that is above the threshold level indicates the subject has both pneumonia and acute decompensated heart failure;
- and,
- (c) setting a treatment regimen or adjusting a treatment regimen for the subject based upon said comparing of the level of the ghrelin signal peptide fragment in the body fluid sample with the reference body fluid sample.
An assay method for diagnosing a subject as having both acute decompensated heart failure and pneumonia, where the subject has i) one or more symptoms of pneumonia, and/or ii) one or more symptoms of acute decompensated heart failure, the assay method comprising:
- (a) contacting a body fluid sample from the subject with an antibody, or antigen-binding antibody fragment thereof, that selectively binds a ghrelin signal peptide (GHRsp) fragment that is (a) GHRsp (1-9) (SEQ ID NO:3);
- or (b) GHRsp (1-10) (SEQ ID NO:4);
- (b) determining a level of ghrelin signal peptide fragment bound by the antibody, or antigen-binding antibody fragment thereof;
- and
- (c) comparing the level of said bound ghrelin signal peptide fragment with a threshold level of the ghrelin signal peptide fragment that distinguishes a population of subjects having both pneumonia and acute decompensated heart failure from a population of subjects not having both pneumonia and acute decompensated heart failure, wherein a level of ghrelin signal peptide fragment that is above the threshold level indicates the subject has both pneumonia and acute decompensated heart failure.
Dependencies are inferred from the claim text.