Combinatorial metabolic engineering of Saccharomyces cerevisiae for terminal alkene production
AssigneeNational University of Singapore
InventorsMatthew Wook Chang, Binbin Chen
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Abstract
Modified Saccharomyces cerevisiae yeast that produce terminal alkenes are described. The modification of the Saccharomyces cerevisiae yeast includes insertion of at least one heterologous fatty acid decarboxylase gene, deletion of FAA1 and FAA4, overexpression of HEM3, and triple-deletion of CTT1, CTA1 and CCP1. Methods of producing terminal alkenes by culturing and fermenting the modified Saccharomyces cerevisiae yeast and optionally harvesting the terminal alkenes are also described. Mixtures of terminal alkenes produced by the modified Saccharomyces cerevisiae yeast, and methods of metabolically engineering a yeast for optimizing overexpression of one or more alkenes are also described.
Claims10
A modified Saccharomyces cerevisiae yeast wherein the modification comprises:
- insertion of at least one heterologous fatty acid decarboxylase gene encoding a fatty acid decarboxylase that synthesizes terminal alkenes selected from 1-undecene, 1-tridecene, 1-pentadecene, 1-heptadecene or 1-nonadecene,
- deletion of fatty acyl-Coenzyme A synthetases, FAA1 and FAA4,
- overexpression of porphobilinogen deaminase, HEM3, and
- triple-deletion of catalase T, CTT1, catalase A CTA1 and cytochrome c peroxidase, CCP1.
Dependencies are inferred from the claim text.