Microorganisms having enhanced L-amino acids productivity and process for producing L-amino acids using the same
AssigneeCj Cheiljedang Corporation
InventorsEun Sung Koh, Su Yon Kwon, Kwang Ho Lee, Ji Sun Lee, Juno Jang, Keun Cheol Lee, Hyeong Pyo Hong
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Abstract
Disclosed are a recombinant microorganism having enhanced L-amino acid productivity, wherein the recombinant microorganism is transformed to have removed or decreased activity of at least one of adenosine deaminase and AMP nucleosidase, and a method of producing an L-amino acid using the recombinant microorganism. The use of the recombinant microorganism may enable the production of the L-amino acid in a highly efficient manner.
Claims11
A recombinant microorganism, wherein activity of at least one of adenosine deaminase comprising the amino acid sequence of SEQ ID NO:
- 14 and AMP nucleosidase comprising the amino acid sequence of SEQ ID NO:
- 16 is removed or decreased compared to that of a mother strain, wherein the recombinant microorganism belongs to the genus Escherichia and has enhanced producibility of an L-amino acid compared to that of the mother strain, wherein the L-amino acid is L-threonine or L-tryptophan, and wherein the recombinant microorganism does not contain chloramphenicol marker gene.
A method of producing L-amino acid, the method comprising:
- culturing a recombinant microorganism;
- and
- collecting an L-amino acid from the culture, wherein the L-amino acid is L-threonine or L-tryptophan,
- wherein the recombinant microorganism has an activity of at least one of adenosine deaminase comprising the amino acid sequence of SEQ ID NO:14 and AMP nucleosidase comprising the amino acid sequence of SEQ ID NO:16 removed or decreased compared to that of a mother strain, does not contain chloramphenical marker gene, and has enhanced producibility of L-threonine or L-tryptophan compared to that of the mother strain.
Dependencies are inferred from the claim text.