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US 10,113,164 B2Granted
Pichia pastoris surface display system
AssigneeResearch Corporation Technologies, Inc.
InventorsKurt R. Gehlsen, Thomas G. Chappell
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Abstract
This disclosure relates to novel Pichia pastoris display systems, e.g., display systems featuring the Pichia pastoris strains (such as SuperMan5) with substantially homogeneous N-glycans displayed on cell surface proteins.
Claims12
A surface display system comprising:
- (a) an engineered strain of Pichia pastoris comprising a mutant α-1,6-mannosyltransferase (OCH1) allele which is transcribed into a mRNA coding for a mutant OCH1 protein, wherein the mutant OCH1 protein comprises a catalytic domain at least 95% identical with amino acids 45-404 of SEQ ID NO:
- 2, wherein the mutant OCH1 protein lacks an N-terminal sequence for targeting the mutant OCH1 protein to the Golgi apparatus and lacks a membrane anchor domain at the N-terminal region, and wherein said strain produce substantially homogeneous N-glycans;
- and
- (b) a vector encoding a recombinant protein adapted to be displayed on a surface of the Pichia pastoris.
1 more element
Dependencies are inferred from the claim text.