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US 10,113,154 B2Granted
Method for producing steviol glycoside
AssigneeSuntory Holdings Limited
InventorEiichiro Ono
Life of this patent
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Abstract
The invention provides a method for producing steviol glycosides. The invention provides a transformant having introduced therein the steviol glucosyltransferase and a method for producing steviol glycosides using the transformant.
Claims18
A polynucleotide selected from:
- a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
- 1;
- and
- a polynucleotide comprising a heterologous regulatory element operably linked to a polynucleotide sequence encoding a protein consisting of the amino acid sequence of SEQ ID NO:
- 2.
2 more elements
A method for producing a steviol glycoside, comprising:
- contacting, outside of a stevia plant, a protein consisting of the amino acid sequence of SEQ ID NO:
- 2 with a UDP-sugar and a compound represented by formula (I):
- wherein R1 represents H, a C1-C20 alkyl, a C2-C20 alkenyl, a C2-C20 alkynyl, a C4-C20 alkyldienyl, a C6-C18 aryl, a C6-C20 alkylaryl, a C6-C20 arylalkyl, a C4-C20 cycloalkyl, a C4-C20 cycloalkenyl, a (C3-C10 cycloalkyl) C1-C10 alkyl, or a sugar residue.
Dependencies are inferred from the claim text.