Human anti-IFN-α antibodies
AssigneeImmunoQure AG
InventorsSyeda F. Y. Haque, Adrian Hayday, Kai Kisand, Kai Krohn, Annalisa Macagno, Steffen Meyer, Paert Peterson, Mike Rothe, Philip Vlaicu, Martin Woodward
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Abstract
Provided are novel IFB-a binding molecules of human origin, particularly human-derived anti-IFN-α antibodies as well as IFN-α binding fragments, derivatives and variants thereof. In addition, pharmaceutical compositions, kits, and methods for use in diagnosis and therapy are described.
Claims34
A human monoclonal anti-interferon-alpha (IFN-α) antibody or an IFN-α binding fragment thereof, wherein the antibody or antigen-binding fragment thereof comprises in its variable regions the complementarily determining regions (CDRs) VH CDRs:
- amino acids 31-35 of SEQ ID NO:18 and amino acids 50-66 of SEQ ID NO:
- 18 and amino acids 99-115 of SEQ ID NO:
- 18;
- and VL CDRs:
- amino acids 24-35 of SEQ ID NO:20 and amino acids 51-57 of SEC) ID NO:20 and amino acids 90-100 of SEC) ID NO:20 and wherein the antibody has the following properties:
- (i) binds to human IFN-α subtypes IFNαI/13 (IFNαIb), IFNα2, IFNα4, IFNα5, IFNα6, IFNα7, IFNα8, IFNα10, IFNα14 IFNα16, IFNα17 and IFNα21;
- and (ii) does not bind to IFN-ω, wherein the IFN-α antibody or IFN-α binding fragment thereof is labeled with a detectable label selected from the group consisting of an enzyme, a radioisotope, a fluorophore, a peptide and a heavy metal, or attached to a drug.
A cDNA molecule encoding a monoclonal anti-interferon-alpha (IFN-α) antibody or an IFN-α binding fragment thereof, wherein the antibody or IFN-α binding fragment thereof comprises in its variable regions the complementarity determining regions (CDRs)
- VH CDRs:
- amino acids 31-35 of SEQ ID NO:18 and amino acids 50-66 of SEQ ID NO:18 and amino acids 99-115 of SEQ ID NO:18;
- and
- VL CDRs:
- amino acids 24-35 of SEQ ID NO:20 and amino acids 51-57 of SEQ ID NO:20 and amino acids 90-100 of SEQ ID NO:20 and wherein the antibody has the following properties
- (i) binds to human IFN-α subtypes IFNα1/13 (IFNα1b), IFNα2, IFNα4, IFNα5, IFNα6, IFNα7, IFNα8, IFNα10, IFNα14, IFNα16, IFNα17 and IFNα21;
- and
- (ii) does not bind to IFN-ω.
A polynucleotide linked to a heterologous nucleic acid, wherein the polynucleotide is selected from the group consisting of:
- (a) a polynucleotide encoding an immunoglobulin heavy chain or a fragment thereof comprising a heavy chain variable region (VH) comprising the complementarity determining regions (CDRs) of the VH variable region amino acid sequence depicted in SEQ ID NO:
- 18, wherein the immunoglobulin heavy chain VH when paired with a light chain variable region (VL) comprising the amino acid sequence depicted in SEQ ID NO:
- 20
- (i) binds to human IFN-α subtypes IFNα1/13 (IFNα1b), IFNα2, IFNα4, IFNα5, IFNα6, IFNα7, IFNα8, IFNα10, IFNα14, IFNα16, IFNα17 and IFNα21;
- and
- (ii) does not bind to IFN-ω;
- (b) a polynucleotide encoding an immunoglobulin light chain or a fragment thereof comprising a light chain variable region (VL) comprising the complementarity determining regions (CDRs) of the VL variable region amino acid sequence depicted in SEQ ID NO:
- 20, wherein the immunoglobulin light chain VL when paired with a heavy chain variable region (VH) comprising the amino acid sequence depicted in SEQ ID NO:
- 18
- i) binds to human IFN-α subtypes IFNα1/13 (IFNα1b), IFNα2, IFNα4, IFNα5, IFNα6, IFNα7, IFNα8, IFNα10, IFNα14, IFNα16, IFNα17 and IFNα21;
- and
- (ii) does not bind to IFN-ω;
- (c) a polynucleotide encoding
- (i) an immunoglobulin heavy chain or a fragment thereof comprising a heavy chain variable region (VH) comprising the complementarity determining regions (CDRs) of the VH variable region amino acid sequence depicted in SEQ ID NO:
- 18;
- and
- (ii) an immunoglobulin light chain or a fragment thereof comprising a light chain variable region (VL) comprising the complementarity determining regions (CDRs) of the VL variable region amino acid sequence depicted in SEQ ID NO:
- 20;
- (d) a polynucleotide encoding an immunoglobulin heavy chain or a fragment thereof comprising the amino acid sequence of SEQ ID NO:
- 18;
- (e) a polynucleotide encoding an immunoglobulin light chain or a fragment thereof comprising the amino acid sequence of SEQ ID NO:
- 20;
- and
- (f) a polynucleotide encoding an immunoglobulin heavy chain or a fragment thereof comprising the amino acid sequence of SEQ ID NO:
- 18 and an immunoglobulin light chain or a fragment thereof comprising the amino acid sequence of SEQ ID NO:
- 20.
Dependencies are inferred from the claim text.