Cell analysis by mass cytometry
AssigneeFluidigm Canada Inc.
InventorVladimir Baranov
Life of this patent
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Abstract
A combination of mutually exclusive cell-based analytical techniques can be applied to the same group of cells for analysis. The same group of cells can be prepared for analysis by each technique resulting with candidate cells targeted for mass cytometry analysis. This configuration allows for the correlation of the information between each technique to produce a matrix of multi dimension of cellular information with the same group of cells.
Claims16
A sample for analysis by laser-ablation-inductively-coupled-mass-cytometry (LA-ICP-MC), the sample comprising:
- a group of cells mounted on a base;
- wherein the group of cells is labeled with more than one type of elemental conjugated antibody;
- wherein each type of antibody is tagged with a distinct elemental tag;
- wherein each type of elemental conjugated antibody is uniquely distinguishable by its distinct elemental tag;
- wherein the group of cells is labeled with a fluorophore;
- and
- wherein the sample is usable with an LA-ICP-MC system for directing laser pulses to the group of cells to generate plumes including the elemental tags, ionizing the plumes, and detecting the elemental tags by mass cytometry.
A method of preparing a sample for analysis by laser-ablation-inductively-coupled-mass-cytometry (LA-ICP-MC), the method comprising:
- mounting a group of cells on a base;
- labeling the group of cells with more than one type of elemental conjugated antibody, wherein each type of antibody is tagged with a distinct elemental tag, and wherein each type of elemental conjugated antibody is uniquely distinguishable by its distinct elemental tag;
- and
- labeling group of cells with a fluorophore;
- wherein the group of cells is usable with an LA-ICP-MC system for directing laser pulses to the group of cells to generate plumes including the elemental tags, ionizing the plumes, and detecting the elemental tags by mass cytometry.
Dependencies are inferred from the claim text.