Method for manufacturing a microbial detection device, microbial detection method, microbial detection kit, and microbial detection device
AssigneeNational Tsing Hua University
InventorsChen-Meng Kuan, Robert S. Langer, Chao-Min Cheng
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Abstract
The present invention provides a method for manufacturing a microbial detection device, microbial detection method, microbial detection kit and microbial detection device. The manufacturing method includes the following steps: defining a sampling portion and a reaction portion on a substrate. Fiber materials are disposed in the reaction portion and a surface of the reaction portion which contacts with the fiber materials comprises abundant hydroxyl groups. Reaction reagents are then added into the fiber materials. An acidic solution is applied to treat the fiber materials and the hydroxyl groups in the reaction portion.
Claims6
A microbial detection method, comprising steps of:
- providing a detection device, wherein the detection device comprises a first substrate, wherein the first substrate comprises a sampling zone and a reacting zone, wherein the reacting zone includes a fiber material and a reacting reagent, wherein the fiber material comprises hydroxyl groups on its surface, and wherein the reacting zone is treated with an acidic solution;
- providing a test sample to contact with the reacting zone and react with the reacting reagent, wherein the test sample contacts with the sampling zone, and the test sample is moved from the sampling zone toward the reacting zone to react with the reacting reagent;
- removing the fiber material;
- and
- adding an alkaline solution to the reacting zone, wherein the fiber material consists of α-cellulose particles.
Dependencies are inferred from the claim text.