Recombinant RSV reporter virus
AssigneeGeorgia State University Research Foundation, Inc.
InventorsRichard K. Plemper, Dan Yan
Life of this patent
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Abstract
An RSV reporter strain that can be used for high-throughput drug discovery is disclosed. Also disclosed is a recombinant RSV vector that contains an RSV genome encoding the disclosed RSV reporter strain operably linked to an expression control sequence. Also disclosed is an infectious RSV virion produce by expression of the disclosed recombinant RSV vector in a host cell. Also disclosed is a recombinant nucleic acid that comprises an RSV F protein having an escape mutation operably linked to a heterologous expression control sequence. Also disclosed are methods of screening for antiviral agents using the disclosed RSV reporter strains.
Claims16
A recombinant respiratory syncytial virus (RSV) vector, comprising an RSV genome encoding an infectious RSV virion operably linked to an expression control sequence, wherein the RSV genome comprises a nucleic acid encoding a fusion (F) protein having a D401E mutation, a D489E mutation, or combination thereof that allows a RSV virus produced by expression of the RSV vector to escape from GPAR-3710 inhibition, wherein the fusion (F) protein mutation positions correspond to SEQ ID NO. 1, wherein the RSV genome further comprises a nucleic acid encoding a luciferase, and wherein the RSV genome further comprises a nucleic acid encoding a small-molecule assisted shutoff (SMASh) tag wherein the SMASh tag comprises a protease cleavage site, hepatitis C virus-derived NS3 protease domain, and a degron domain.
A recombinant nucleic acid, comprising a respiratory syncytial virus (RSV) fusion (F) protein having a D401E mutation, a D489E mutation, or a combination thereof, operably linked to a heterologous expression control sequence, wherein the D401E mutation, the D489E mutation, or combination thereof allows an RSV virus produced by expression of the recombinant nucleic acid to escape from GPAR-3710 inhibition wherein the RSV F protein mutation positions correspond to SEQ ID NO. 1.
Dependencies are inferred from the claim text.