Cervical cancer-related HPV E7 protein monoclonal antibody and use thereof
AssigneeAttogen Biomedical (Suzhou) Inc. Ltd.
InventorsXiaojia Chang, Lijun Shi, Chenglong Shi, Fengli Han, Yan Huang
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Abstract
The present invention provides an anti-HPV E7 protein monoclonal antibody and the use thereof. The antibody can detect the HPV16 E7 protein with high specificity and recognize the HPV18 E7 protein, thereby it can distinguish between the cancerous cervical epithelial cells and the cervical abnormal or non-cancerous cervical epithelial cells.
Claims15
A variable region of an anti-HPV E7 protein antibody, wherein the variable region is a heavy chain variable region comprising three complementary determining regions:
- CDR1 as shown in SEQ ID NO:
- 4;
- CDR2 as shown in SEQ ID NO:
- 6;
- and
- CDR3 as shown in SEQ ID NO:
- 8, or
- the variable region is a light chain variable region comprising three complementary determining regions:
- CDR1′ as shown in SEQ ID NO:
- 14;
- CDR2′ as shown in SEQ ID NO:
- 16;
- and
- CDR3′ as shown in SEQ ID NO:
- 18.
A recombinant protein having:
- (1) a heavy chain variable region comprising three complementary determining regions:
- CDR1 as shown in SEQ ID NO:
- 4;
- CDR2 as shown in SEQ ID NO:
- 6;
- and
- CDR3 as shown in SEQ ID NO:
- 8, and/or
- (2) a light chain variable region comprising three complementary determining regions:
- CDR1′ as shown in SEQ ID NO:
- 14;
- CDR2′ as shown in SEQ ID NO:
- 16;
- and
- CDR3′ as shown in SEQ ID NO:
- 18.
A method to detect a HPV E7 protein in a sample, wherein the method comprises steps of:
- (1) contacting a sample with an antibody wherein the antibody having:
- (a) a heavy chain variable region comprising three complementary determining regions:
- CDR1 as shown in SEQ ID NO:
- 4;
- CDR2 as shown in SEQ ID NO:
- 6;
- and
- CDR3 as shown in SEQ ID NO:
- 8;
- and
- (b) a light chain variable region comprising three complementary determining regions:
- CDR1′ as shown in SEQ ID NO:
- 14;
- CDR2′ as shown in SEQ ID NO:
- 16;
- and
- CDR3′ as shown in SEQ ID NO:
- 18, and
- (2) detecting whether an antigen-antibody complex is formed, wherein the formation of the antigen-antibody complex indicates a presence of a HPV E7 protein in the sample.
Dependencies are inferred from the claim text.