Norovirus detection sensor and electrochemical sensing method using the same

A norovirus detection sensor comprising:

• a bioreceptor;
• and
• a signal detector,
• wherein
• the surface of bioreceptor comprises a three-dimensional gold nanosurface electrode substrate,
• the three-dimensional gold nanosurface electrode substrate is covered with
• a protein having a specific binding property with the norovirus and
• a mercaptohexanol preventing non-specific binding to the substrate,
• the protein is concanavalin A immobilized on the three-dimensional gold nanosurface electrode substrate as a sample capture agent for causing the concanavalin A to bind to the norovirus, and
• the mercaptohexanol is formed, by drop-casting, in an exposed area of the three-dimensional gold nanosurface electrode substrate in which the concanavalin A is not immobilized to serve to cover the exposed are,
• the signal detector detects electrochemical signal generated from the bioreceptor, and qualitatively and quantitatively calculates, from the detected electrochemical signal, values of the biological sample, and
• the norovirus detection sensor has a limit of detection (LOD) ranging from 35 copies/mL to 60 copies/mL and a detection selectivity for norovirus of 98% or more.