Strip for monitoring analyte concentrations

A method for measuring the concentration of at least one analyte C in a liquid sample, said method comprising:

• i) applying a liquid sample to an application zone, the application zone comprising at least one molecule A capable of binding specifically to an analyte C, said at least one specific molecule A being coupled to a reporter D, said at least one molecule A not being immobilised on the surface of the application zone, so that analyte C from the sample is bound by the at least one specific molecule A conjugated to the reporter D, and wherein said reporter D is capable of affecting electrical properties;
• ii) allowing migration of analyte C contained in the application zone to at least one detection zone comprising at least one molecule B capable of binding specifically to said analyte C, said at least one molecule B being immobilised on the surface of the at least one detection zone, wherein the at least one specific molecule A and the at leak one specific molecule B can bind the analyte C simultaneously;
• iii) wherein the at least one immobilised molecule B capable of binding specifically the analyte C will retain the analyte C conjugated to the reporter D;
• and
• iv) measuring induced changes in electrical properties in the at least one detection zone by using at least two electrodes in ohmic contact with the detection zone thereby providing direct electrical contact with the liquid sample, wherein said changes in electrical properties are a function of the concentration of said analyte.