Specific analysis of ketone and aldehyde analytes using reagent compounds, labeling strategies, and mass spectrometry workflow
AssigneeDH Technologies Development Pte. Ltd.
InventorsSubhakar Dey, Sasi Pillai, Brian L. Williamson, Subhasish Purkayastha, Michal Weinstock
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Abstract
Labeling reagents, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids and includes testosterone. The analytes can be medical or pharmaceutical compounds in biological samples. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.
Claims20
A method for mass analysis of a ketosteroid in a sample, the ketosteroid comprising an aldehyde or ketone functional group, the method comprising:
- derivatizing a ketosteroid comprising an aldehyde or ketone functional group, with a labeling reagent of formula:
- Y—(CH2)n—ONH2
- to form a labeled ketosteroid,
- wherein n is an integer from 2 to 10 and Y is a group selected from the group consisting of:
- subjecting the labeled ketosteroid to ionization;
- and
- detecting the ketosteroid by mass analysis.
A method for mass analysis of testosterone in a sample comprising:
- derivatizing the testosterone in the sample with a labeling reagent of formula:
- Y—(CH2)n—ONH2
- to form a labeled testosterone,
- wherein n is an integer from 2 to 6 and Y is a group selected from the group consisting of:
- subjecting the labeled testosterone to ionization;
- and
- detecting the testosterone by mass analysis.
Dependencies are inferred from the claim text.