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US 10,094,031 B2Granted
Method for manufacturing reduced glutathione
AssigneeKyowa Hakko Bio Co., Ltd.
InventorsKenta Fukumoto, Miyuki Fukuda, Mitsutaka Kino
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Abstract
A method of producing reduced glutathione by electrolytic reduction of oxidized glutathione using a cathode cell and an anode cell separated from each other by a separating membrane, including using a cathode having a metal cathode surface, and, as a cathode solution, an aqueous oxidized glutathione solution having a pH adjusted to higher than 3.0 and 5.0 or below by adding a base, which is added with the same metal as the metal of the cathode surface, a metal salt thereof, or a metal oxide thereof.
Claims16
A method of producing reduced glutathione comprising electrolytically reducing oxidized glutathione in a cathode solution to produce reduced glutathione in a system comprising
- (a) a cathode cell comprising a cathode having a metal cathode surface, wherein the metal on the cathode surface is a metal showing high hydrogen overvoltage,
- (b) an anode cell comprising an anode,
- (c) a membrane separating the cathode cell and the anode cell, and
- (d) the cathode solution that is an aqueous oxidized glutathione solution comprising, dissolved therein, a metal-containing compound, a metal salt, or a metal oxide,
- wherein the metal of the metal-containing compound, the metal salt, or the metal oxide is the same metal as the metal of the cathode surface, and
- wherein the aqueous oxidized glutathione solution has a pH adjusted to be higher than 3.0 and up to 5.0 by adding a base thereto.
3 more elements
A method of producing a reduced glutathione crystal, comprising
- electrolytically reducing oxidized glutathione in a cathode solution to produce reduced glutathione in a system comprising
- (a) a cathode cell comprising a cathode having a metal cathode surface, wherein the metal on the cathode surface is a metal showing high hydrogen overvoltage,
- (b) an anode cell comprising an anode,
- (c) a membrane separating the cathode cell and the anode cell, and
- (d) the cathode solution that is an aqueous oxidized glutathione solution comprising, dissolved therein, a metal-containing compound, a metal salt, or a metal oxide,
- wherein the metal of the metal-containing compound, the metal salt, or the metal oxide is the same metal as the metal of the cathode surface, and
- wherein the aqueous oxidized glutathione solution has a pH adjusted to be higher than 3.0 and up to 5.0 by adding a base thereto,
- then subjecting the cathode solution comprising reduced glutathione to 1) adjustment of pH, or 2) removal of cation by passing the cathode solution through an ion exchange column, and
- then crystallizing the reduced glutathione from the cathode solution to provide a reduced glutathione crystal.
6 more elements
A method of producing a reduced glutathione crystal, comprising
- electrolytically reducing oxidized glutathione in a cathode solution to produce reduced glutathione in a system comprising
- (a) a cathode cell comprising a cathode having a metal cathode surface, wherein the metal on the cathode surface is a metal showing high hydrogen overvoltage,
- (b) an anode cell comprising an anode,
- (c) a membrane separating the cathode cell and the anode cell, and
- (d) the cathode solution that is an aqueous oxidized glutathione solution comprising, dissolved therein, a metal-containing compound, a metal salt, or a metal oxide,
- wherein the metal of the metal-containing compound, the metal salt, or the metal oxide is the same metal as the metal of the cathode surface, and
- wherein the aqueous oxidized glutathione solution has a pH adjusted to be higher than 3.0 and up to 5.0 by adding a base thereto selected from sodium or potassium hydroxide, sodium or potassium carbonate, and sodium or potassium hydrogen carbonate,
- then subjecting the cathode solution comprising reduced glutathione to 1) adjustment of pH, or 2) removal of cation by passing the cathode solution through an ion exchange column, and
- then crystallizing the reduced glutathione from the cathode solution to provide a reduced glutathione crystal.
6 more elements
A method of producing a reduced glutathione crystal, comprising
- electrolytically reducing oxidized glutathione in a cathode solution to produce reduced glutathione in a system comprising
- (a) a cathode cell comprising a cathode having a metal cathode surface, wherein the metal on the cathode surface is a metal showing high hydrogen overvoltage,
- (b) an anode cell comprising an anode,
- (c) a membrane separating the cathode cell and the anode cell, and
- (d) the cathode solution that is an aqueous oxidized glutathione solution comprising, dissolved therein, a metal-containing compound, a metal salt, or a metal oxide,
- wherein the cathode solution comprises oxidized glutathione at a concentration of not less than 20 g/L,
- wherein the metal of the metal-containing compound, the metal salt, or the metal oxide is the same metal as the metal of the cathode surface, and
- wherein the aqueous oxidized glutathione solution has a pH adjusted to be higher than 3.0 and up to 5.0 by adding a base thereto,
- then subjecting the cathode solution comprising reduced glutathione to 1) adjustment of pH, or 2) removal of cation by passing the cathode solution through an ion exchange column, and
- then crystallizing the reduced glutathione from the cathode solution to provide a reduced glutathione crystal.
7 more elements
A method of producing a reduced glutathione crystal, comprising
- electrolytically reducing oxidized glutathione in a cathode solution to produce reduced glutathione in a system comprising
- (a) a cathode cell comprising a cathode having a metal cathode surface, wherein the metal on the cathode surface is a metal showing high hydrogen overvoltage,
- (b) an anode cell comprising an anode,
- (c) a membrane separating the cathode cell and the anode cell, and
- (d) the cathode solution that is an aqueous oxidized glutathione solution comprising, dissolved therein, at a concentration of 0.5-50 mmol/L, one of a metal-containing compound, a metal salt, or a metal oxide,
- wherein the metal of the metal-containing compound, the metal salt, or the metal oxide is the same metal as the metal of the cathode surface, and
- wherein the aqueous oxidized glutathione solution has a pH adjusted to be higher than 3.0 and up to 5.0 by adding a base thereto,
- then subjecting the cathode solution comprising reduced glutathione to 1) adjustment of pH, or 2) removal of cation by passing the cathode solution through an ion exchange column, and
- then crystallizing the reduced glutathione from the cathode solution to provide a reduced glutathione crystal.
6 more elements
A method of producing a reduced glutathione crystal, comprising
- electrolytically reducing oxidized glutathione in a cathode solution to produce reduced glutathione in a system comprising
- (a) a cathode cell comprising a cathode having a metal cathode surface, wherein the metal on the cathode surface is a metal showing high hydrogen overvoltage,
- (b) an anode cell comprising an anode,
- (c) a membrane separating the cathode cell and the anode cell, and
- (d) the cathode solution that is an aqueous oxidized glutathione solution comprising, dissolved therein, a metal-containing compound, a metal salt, or a metal oxide,
- wherein the metal of the metal-containing compound, the metal salt, or the metal oxide is the same metal as the metal of the cathode surface,
- wherein the aqueous oxidized glutathione solution has a pH adjusted to be higher than 3.0 and up to 5.0 by adding a base thereto, and
- wherein the electrolytic reduction is performed at an electric current density of 0.1-30 A/dm2,
- then subjecting the cathode solution comprising reduced glutathione to 1) adjustment of pH, or 2) removal of cation by passing the cathode solution through an ion exchange column, and
- then crystallizing the reduced glutathione from the cathode solution to provide a reduced glutathione crystal.
7 more elements
A method of producing a reduced glutathione crystal, comprising
- electrolytically reducing oxidized glutathione in a cathode solution to produce reduced glutathione in a system comprising
- (a) a cathode cell comprising a cathode having a metal cathode surface, wherein the metal on the cathode surface is a metal showing high hydrogen overvoltage,
- (b) an anode cell comprising an anode,
- (c) a membrane separating the cathode cell and the anode cell, and
- (d) the cathode solution that is an aqueous oxidized glutathione solution comprising, dissolved therein, a metal-containing compound, a metal salt, or a metal oxide,
- wherein the cathode solution comprises oxidized glutathione at a concentration of not less than 20 g/L,
- wherein the cathode solution comprises the metal-containing compound, metal salt, or metal oxide at a concentration of 0.5-50 mmol/L,
- wherein the metal of the metal-containing compound, the metal salt, or the metal oxide is the same metal as the metal of the cathode surface,
- wherein the aqueous oxidized glutathione solution has a pH adjusted to be higher than 3.0 and up to 5.0 by adding a base thereto,
- wherein the base is hydroxide, carbonate or hydrogen carbonate of sodium or potassium,
- wherein the electrolytic reduction is performed at an electric current density of 0.1-30 A/dm2,
- then subjecting the cathode solution comprising reduced glutathione to 1) adjustment of pH, or 2) removal of cation by passing the cathode solution through an ion exchange column, and
- then crystallizing the reduced glutathione from the cathode solution to provide a reduced glutathione crystal.
10 more elements
Dependencies are inferred from the claim text.