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US 10,093,991 B2Granted
Compositions and methods for the detection of the shrunken2-R mutation in maize
AssigneeSyngenta Participations AG
InventorVance Cary Kramer
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Abstract
The present invention relates to compositions and methods for detecting the shrunken2-R (sh2-R) mutation and identifying maize plants, maize plant parts and/or maize germplasm having the sh2-R mutation.
Claims12
A method of identifying a maize plant, plant part or plant cell having a sh2-R mutation, the method comprising:
- (a) isolating a nucleic acid from a maize plant, plant part or plant cell;
- (b) amplifying in said nucleic acid a region of a sh2-R nucleotide sequence, wherein said region comprises a junction between a sh2 gene and an insertion of the 3′ end of the sh2-R nucleotide sequence, wherein said junction corresponds to nucleotides 69686-69687 of SEQ ID NO:
- 32;
- and
- c) analyzing the amplification reaction for the presence of an amplification product, thereby identifying a maize plant, plant part or plant cell having a sh2-R mutation.
2 more elements
A method of reducing the presence of, or eliminating, the sh2-R mutation from a maize population, the method comprising:
- (a) isolating a nucleic acid from a maize plant or a plant part or plant cell thereof;
- (b) amplifying in said nucleic acid a region of a sh2-R nucleotide sequence, wherein said region comprises a junction between a sh2 gene and an insertion of the 3′ end of the sh2-R nucleotide sequence, wherein said junction corresponds to nucleotides 69686-69687 of SEQ ID NO:
- 32;
- c) analyzing the amplification reaction for the presence of an amplification product, thereby identifying a maize plant having a sh2-R mutation;
- and
- (d) removing said maize plant from the population, thereby reducing the presence of, or eliminating, the sh2-R mutation from the maize population.
3 more elements
Dependencies are inferred from the claim text.