Red blood cell lysis solution
AssigneeGen-Probe Incorporated
InventorsJijumon Chelliserry, Kui Gao, Jeffrey M. Linnen
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Abstract
The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.
Claims20
A method of analyzing a target RNA from red blood cells comprising:
- (a) contacting red blood cells with a reagent comprising ammonium chloride at a concentration of 200-300 mM and lithium lauryl sulfate (LLS) at a concentration of 4% to 15% (w/v), the reagent being effective to lyse the red blood cells and inhibit degradation of target RNA released from the red blood cells;
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- (b) analyzing the target RNA released from the red blood cells, wherein said analyzing comprises performing an amplification reaction to amplify the target RNA.
A method of analyzing a target RNA from red blood cells comprising:
- (a) contacting red blood cells with a reagent comprising ammonium chloride at a concentration of 100-500 mM, lithium lauryl sulfate (LLS) at a concentration of 4% to 15% (w/v), and sodium bicarbonate buffer at a concentration of 10-20 mM and at pH 7-8, the reagent being effective to lyse the red blood cells and inhibit degradation of target RNA released from the red blood cells;
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- (b) analyzing the target RNA released from the red blood cells, wherein said analyzing comprises performing an amplification reaction to amplify the target RNA.
Dependencies are inferred from the claim text.