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US 10,093,987 B2Granted
Multiplexed KRAS mutation detection assay
AssigneeExact Sciences Development Company, LLC
InventorsRebecca Oldham-Haltom, Hatim Allawi, Hongzhi Zou, Michael J. Domanico, Graham P. Lidgard
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Abstract
Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.
Claims12
A kit comprising:
- at least seven different primers that each comprise, at their 3′ end, at least 12 contiguous nucleotides starting from the 3′ end of a sequence selected from SEQ ID NOs:
- 1-7, wherein the primers are in one or more containers.
Dependencies are inferred from the claim text.