Leukemia methylation markers and uses thereof
AssigneesYouhealth Biotech, Limited, The Regents of the University of California
InventorsKang Zhang, Rui Hou, Lianghong Zheng
Life of this patent
Estimating the term…
Abstract
Disclosed herein are methods and kits for identifying a subject as having leukemia. Also provided herein are methods and kits for determining a leukemia subtype in subject. Further provided herein are methods and kits for determining the prognosis of a subject having leukemia and for determining the progression of leukemia in a subject.
Claims21
A method of detecting a methylation pattern of a set of biomarkers in a subject suspected of having leukemia, the method comprising:
- a) processing an extracted genomic DNA with a deaminating agent to generate a genomic DNA sample comprising deaminated nucleotides, wherein the extracted genomic DNA is obtained from a biological sample from the subject suspected of having leukemia;
- and
- b) detecting the methylation pattern of one or more biomarkers selected from:
- cg08261841, cg09247255, cg00142402, cg16274678, and cg02381853 from the extracted genomic DNA by contacting the extracted genomic DNA with a set of probes, wherein the set of probes hybridizes to the one or more biomarkers, and perform a DNA sequencing analysis to determine the methylation pattern of the one or more biomarkers.
A method of detecting a methylation pattern of a set of biomarkers in a subject suspected of having acute lymphoblastic leukemia (ALL), comprising:
- a) processing an extracted genomic DNA with a deaminating agent to generate a genomic DNA sample comprising deaminated nucleotides, wherein the extracted genomic DNA is obtained from a biological sample from the subject;
- and
- b) detecting the methylation pattern of biomarkers cg08261841, cg09247255, cg12008047, cg05304729, cg18518074, cg05048927, and cg08960448 from the extracted genomic DNA by contacting the extracted genomic DNA with a set of probes, wherein the set of probes hybridizes to the one or more biomarkers, and perform a DNA sequencing analysis to determine the methylation pattern of the one or more biomarkers.
A method of detecting a methylation pattern of a set of biomarkers in a subject suspected of having acute myeloid leukemia (AML), comprising:
- a) processing an extracted genomic DNA with a deaminating agent to generate a genomic DNA sample comprising deaminated nucleotides, wherein the extracted genomic DNA is obtained from a biological sample from the subject;
- and
- b) detecting the methylation pattern of biomarkers cg00142402, cg05304729, cg00484711, and cg18518074 from the extracted genomic DNA by contacting the extracted genomic DNA with a set of probes, wherein the set of probes hybridizes to the one or more biomarkers, and perform a DNA sequencing analysis to determine the methylation pattern of the one or more biomarkers.
Dependencies are inferred from the claim text.