Methods for detecting gene dysregulation by intragenic differential expression
AssigneeQuest Diagnostics Investments Incorporated
InventorsHeather R. Sanders, Maher Albitar, Aurelia Meloni-Ehrig
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Abstract
Described herein are methods, compositions and kits directed to the detection of gene dysregulations such as those arising from gene fusions and/or chromosomal abnormalities, e.g., translocations, insertions, inversions and deletions. Samples containing dysregulated gene(s) of interest may show independent expression patterns for the 5′ and 3′ regions of the gene. The methods, compositions and kits are useful for detecting mutations that cause the differential expression of a 5′ portion of a target gene relative to the 3′ region of the target gene.
Claims9
A kit comprising:
- (a) a 5′ target primer pair that is complementary to a 5′ region of a TMPRSS2 gene transcript, wherein a first primer of the 5′ target primer pair comprises the sequence of SEQ ID NO:
- 1 and a second primer of the 5′ target primer pair comprises the sequence of SEQ ID NO:
- 2;
- (b) an oligonucleotide probe that is complementary to an amplification product generated using the 5′ target primer pair, and labeled with a donor fluorophore and a quenching moiety;
- (c) a 3′ target primer pair that is complementary to a 3′ region of the TMPRSS2 gene transcript, wherein a first primer of the 3′ target primer pair comprises the sequence of SEQ ID NO:
- 5 and a second primer of the 3′ target primer pair comprises the sequence of SEQ ID NO:
- 6;
- and
- (d) an oligonucleotide probe that is complementary to an amplification product generated using the 3′ target primer pair, and labeled with a donor fluorophore and a quenching moiety.
A kit comprising:
- (a) a 5′ target primer pair that is complementary to a 5′ region of a ERG gene transcript wherein a first primer of the 5′ target primer pair comprises SEQ ID NO:
- 7 and a second primer of the 5′ target primer pair comprises SEQ ID NO:
- 8;
- (b) an oligonucleotide probe that is complementary to an amplification product generated using the 5′ target primer pair, and labeled with a donor fluorophore and a quenching moiety;
- (c) a 3′ target primer pair that is complementary to a 3′ region of the ERG gene transcript wherein a first primer of the 3′ target primer pair comprises SEQ ID NO:
- 10 and a second primer of the 3′ target primer pair comprises SEQ ID NO:
- 11;
- and
- (d) an oligonucleotide probe that is complementary to an amplification product generated using the 3′ target primer pair, and labeled with a donor fluorophore and a quenching moiety.
A kit comprising:
- (a) a 5′ target primer pair that is complementary to a 5′ region of a ALK gene transcript wherein a first primer of the 5′ target primer pair comprises the sequence of SEQ ID NO:
- 13 and a second primer of the 5′ target primer pair comprises the sequence of SEQ ID NO:
- 14;
- (b) an oligonucleotide probe that is complementary to an amplification product generated using the 5′ target primer pair, and labeled with a donor fluorophore and a quenching moiety;
- (c) a 3′ target primer pair that is complementary to a 3′ region of the ALK gene transcript wherein a first primer of the 3′ target primer pair comprises the sequence of SEQ ID NO:
- 16 and a second primer of the 3′ target primer pair comprises the sequence of SEQ ID NO:
- 17;
- and
- (d) an oligonucleotide probe that is complementary to an amplification product generated using the 3′ target primer pair, and labeled with a donor fluorophore and a quenching moiety.
Dependencies are inferred from the claim text.