Method for improving nucleic acid synthesis reaction
AssigneeTakara Bio Inc.
InventorsKiyoyuki Matsumura, Takashi Uemori, Hiroyuki Mukai
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Abstract
Provided are the following: a method, for improving reactivity of an acid synthesis reaction, comprising a step for adding an ω-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleoside triphosphate, and an ω-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ω-amino acid.
Claims5
A composition for a nucleic acid synthesis reaction, containing:
- a DNA polymerase,
- a reaction buffering agent,
- at least one primer,
- at least one deoxyribonucleoside triphosphate, and
- an ω-amino acid
- wherein the ω-amino acid is selected from the group consisting of β-alanine, δ-aminopentanoic acid (5-aminopentanoic acid), ε-aminohexanoic acid (6-aminohexanoic acid), ω-aminoheptanoic acid (7-aminoenanthic acid), 8-aminooctanoic acid, 9-aminononanoic acid, and 10-aminodecanoic acid.
A reaction buffer for a nucleic acid synthesis reaction, containing
- betaine, and
- an ω-amino acid
- wherein the ω-amino acid is selected from the group consisting of β-alanine, δ-aminopentanoic acid (5-aminopentanoic acid), ε-aminohexanoic acid (6-aminohexanoic acid), ω-aminoheptanoic acid (7-aminoenanthic acid), 8-aminooctanoic acid, 9-aminononanoic acid, and 10-aminodecanoic acid.
A kit for a nucleic acid synthesis reaction, comprising the following components:
- a DNA polymerase,
- a reaction buffering agent,
- at least one deoxyribonucleoside triphosphate, and
- an ω-amino acid
- wherein the ω-amino acid is selected from the group consisting of β-alanine, δ-aminopentanoic acid (5-aminopentanoic acid), ε-aminohexanoic acid (6-aminohexanoic acid), ω-aminoheptanoic acid (7-aminoenanthic acid), 8-aminooctanoic acid, 9-aminononanoic acid, and 10-aminodecanoic acid.
Dependencies are inferred from the claim text.