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US 10,093,962 B2Granted
Fast hybridization for next generation sequencing target enrichment
AssigneeAgilent Technologies, Inc.
InventorMichael Borns
Life of this patent
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Abstract
The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.
Claims11
A composition for nucleic acid hybridization, comprising
- a salt of a divalent cation that has a concentration in the range from about 100 mM to about 600 mM,
- a buffering agent, wherein said salt and said buffering agent are present in a molar ratio of about 2.5:1 to about 60:1, and
- a volume-excluding/thickening agent that (i) is selected from the group consisting of hydroxypropyl methyl cellulose (HPMC), hydroxyethyl methyl cellulose (HEMC), hydroxybutyl methyl cellulose, hydroxypropyl cellulose, methyl cellulose, and hydroxyl methyl cellulose and (ii) has a concentration in the range from about 0.002% to about 0.1% w/w.
Dependencies are inferred from the claim text.