Method and apparatus for antibody production and purification

An automated cell culture and purification apparatus for the production of cells and cell derived products, comprising:

• (a) a cell culture unit comprising:
• a first reusable instrumentation base device incorporating hardware comprising a pump;
• and
• at least one first disposable cell cultureware module removably attachable to said first instrumentation base device, wherein said first cultureware module includes a cell growth chamber having ports for movement of cell culture medium through said cell growth chamber, wherein said pump circulates cell culture medium into and out of said cell growth chamber through said ports of said cell growth chamber;
• and
• (b) a purification unit connected to said cell culture unit by a fluid flow path there between, said purification unit comprising:
• a second reusable instrumentation base device incorporating hardware to receive fluid from said cell growth chamber of said cell culture unit through said fluid flow path, wherein said hardware comprises dual pumps for chromatography, comprising a first pump and a second pump, and a plurality of valves;
• and
• at least one second disposable cell cultureware module removably attachable to said second instrumentation base device of said purification unit, said at least one second cultureware module including a chromatography device, a diafiltration module that utilizes tangential flow filtration, an anion exchange filter, and a size-exclusion virus filter, wherein each of said first and second pumps pump fluid to said chromatography device, wherein said chromatography device, said diafiltration module, said size-exclusion virus filter, and said anion exchange filter are connected to said cell growth chamber of said cell culture unit by said fluid flow path, wherein said chromatography device separates a component from fluid received from said cell growth chamber, wherein said diafiltration module separates a component from fluid received from said chromatography device, wherein said anion exchange filter separates a component from fluid received from said diafiltration module or said chromatography device, and wherein said size-exclusion virus filter separates virus from fluid received from said chromatography device, said diafiltration module, or said anion exchange filter.