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US 10,093,955 B2Granted
Acoustic energy mediation of genetic fragmentation
AssigneeCovaris, Inc.
InventorsJames A. Laugharn, Jr., Hamid Khoja
Life of this patent
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Abstract
Method and apparatus for controlling acoustic treatment of a sample to mediate a tagmentation process used on double stranded DNA.
Claims16
A method for processing a sample containing genomic material, comprising:
- providing a sample in a vessel, the sample including double stranded DNA material with segments having a base pair length in excess of a starting base pair length;
- subjecting the sample to acoustic energy to cause random shearing of the segments of double stranded DNA material in the sample to form fragments of the double stranded DNA material having a base pair length of a final base pair length, the starting base pair length being two or more times the final base pair length;
- and
- providing a hyperactive mutant of Tn5 transposase and oligonucleotide material with the fragments of double stranded DNA material to cut the fragments of double stranded DNA material with the Tn5 transposase and insert oligonucleotide material into the fragments of double stranded DNA material at areas cut by the Tn5 transposase.
1 more element
A method for processing a sample containing genomic material, comprising:
- providing a sample in a vessel, the sample including double stranded DNA material with segments having a base pair length in excess of 3000 bp;
- subjecting the sample to acoustic energy to cause random shearing of the segments of double stranded DNA material in the sample to form fragments of the double stranded DNA material having a base pair length less than 3000 bp;
- and
- providing a hyperactive mutant of Tn5 transposase and oligonucleotide material with the fragments of double stranded DNA material to cut the fragments of double stranded DNA material with the Tn5 transposase and insert oligonucleotide material into the fragments of double stranded DNA material at areas cut by the Tn5 transposase, wherein a concentration of the Tn5 transposase is less than that needed to perform a tagmentation process using techniques without acoustic energy treatment.
1 more element
A method for processing a sample containing genomic material, comprising:
- providing a sample in a vessel, the sample including double stranded DNA material with segments having a base pair length in excess of 3000 bp;
- subjecting the sample to acoustic energy to cause random shearing of the segments of double stranded DNA material in the sample to form fragments of the double stranded DNA material having a base pair length less than 3000 bp;
- and
- providing a hyperactive mutant of Tn5 transposase and oligonucleotide material with the fragments of double stranded DNA material to cut the fragments of double stranded DNA material with the Tn5 transposase and insert oligonucleotide material into the fragments of double stranded DNA material at areas cut by the Tn5 transposase, wherein the fragments of double stranded DNA material having inserted oligonucleotide material have a tighter size distribution than that resulting from standard tagmentation processes performed in the absence of acoustic treatment.
1 more element
Dependencies are inferred from the claim text.