Method for the spatial arrangement of sample fragments for amplification and immobilization for further derivatizations

A method for amplifying more than one sample fragment, comprising:

• a) supplying a solid phase with a microstructure, wherein the microstructure subdivides the solid phase so that several compartments are formed, and wherein capture molecules are attached to surfaces in the compartments,
• b) introducing exactly one type of sample fragment into each of the several compartments, wherein each of the sample fragments has an adapters on one or both ends, wherein the adapters have a uniform sequence with a length of 1 to 500 nucleotides and are primers or have a binding site for RNA polymerase or a cell free expression system,
• c) introducing a reaction mixture for amplification into the several compartments,
• d) separating and/or covering the individual compartments to obtain sealed compartments, and
• e) amplifying each sample fragment of said sample fragments independently in the corresponding sealed compartment to obtain an amplification product for each of the sample fragments in each of the compartments,
• f) wherein the amplification products generated by the amplifying in (e) are immobilized on the capture molecules attached to the surfaces in each of the compartments and are subsequently analyzed, sequenced and/or derivatized.