Method for preparing yeast beta-D-glucan using solubilization technology based on molecular assembly

A method for preparing yeast beta-D-glucan using a solubilization technology based on molecular assembly, characterized in that the method consists of the following steps:

• (I) washing the cell walls of Saccharomyces cerevisiae as raw materials with water and then centrifuging to remove the impurities, adding water to prepare a suspension, adding NaCl, placing in a constant temperature water bath oscillator under the conditions of pH 5.0 and 55° C. and autolyzing by induction for 24 h, heating to 85° C., keeping the temperature for 15 min to inactivate an enzyme, centrifuging, and washing the centrifugate with water for 3 times;
• (II) mixing yeast cell walls with water to obtain a suspension, and then centrifuging and collecting a centrifugate;
• (III) resuspending the centrifugate obtained in step (II) with water, extracting by heat, and then centrifuging and collecting the centrifugate;
• (IV) resuspending the centrifugate obtained in step (III) with water, adding a mixed enzyme comprising an endoglucanase, exoglucanase, and β-glucosidase or a neutral protease to enzymatically hydrolyze so as to obtain an enzymatic hydrolysate;
• (V) micro-fluidizing the enzymatic hydrolysate of yeast cell walls prepared in step (IV) at 70 to 200 MPa, and then centrifuging to obtain a centrifugate;
• (VI) resuspending the centrifugate obtained in step (V) with an ionic liquid, then dispersing to obtain a solution;
• wherein the ionic liquid is 1-ethyl-3-methylimidazolium acetate or 1-allyl-3-methylimidazolium chloride;
• (VII) centrifuging the solution obtained in step (VI), and then adding ethanol, and collecting a centrifugate after recentrifuging;
• and
• (VIII) resuspending the centrifugate obtained after recentrifuging in step (VII) with water, and then centrifuging and collecting a supernatant.

A method for preparing yeast beta-D-glucan using a solubilization technology based on molecular assembly, characterized in that the method consists of the following steps:

• (I) mixing yeast cell walls with water to obtain a suspension, and then centrifuging and collecting a centrifugate;
• (II) resuspending the centrifugate obtained in step (I) with water, extracting by heat, and then centrifuging and collecting the centrifugate;
• (III) resuspending the centrifugate obtained in step (II) with water, adding a mixed enzyme comprising an endoglucanase, exoglucanase, and β-glucosidase or a neutral protease to enzymatically hydrolyze so as to obtain an enzymatic hydrolysate;
• (IV) micro-fluidizing the enzymatic hydrolysate of yeast cell walls prepared in step (III) at 70 to 200 MPa, and then centrifuging to obtain a centrifugate;
• (V) resuspending the centrifugate obtained in step (IV) with an ionic liquid, then dispersing to obtain a solution;
• wherein the ionic liquid is 1-ethyl-3-methylimidazolium acetate or 1-allyl-3-methylimidazolium chloride;
• (VI) centrifuging the solution obtained in step (V), and then adding ethanol, and collecting a centrifugate after recentrifuging;
• (VII) resuspending the centrifugate obtained after recentrifuging in step (VI) with water, and then centrifuging and collecting a supernatant;
• and
• (VIII) spray drying the supernatant obtained in step (VII) to obtain a yeast beta-D-glucan powder.

A method for preparing yeast beta-D-glucan using a solubilization technology based on molecular assembly, characterized in that the method consists of the following steps:

• (I) mixing yeast cell walls with water to obtain a suspension, and then centrifuging and collecting a centrifugate;
• (II) resuspending the centrifugate obtained in step (I) with water, extracting by heat, and then centrifuging and collecting the centrifugate;
• (III) resuspending the centrifugate obtained in step (II) with water, adding a mixed enzyme comprising an endoglucanase, exoglucanase, and β-glucosidase or a neutral protease to enzymatically hydrolyze so as to obtain an enzymatic hydrolysate;
• (IV) micro-fluidizing the enzymatic hydrolysate of yeast cell walls prepared in step (III) at 70 to 200 MPa, and then centrifuging to obtain a centrifugate;
• (V) resuspending the centrifugate obtained in step (IV) with an ionic liquid, then dispersing to obtain a solution;
• wherein the ionic liquid is 1-ethyl-3-methylimidazolium acetate or 1-allyl-3-methylimidazolium chloride;
• (VI) centrifuging the solution obtained in step (V), and then adding ethanol, and collecting a centrifugate after recentrifuging;
• washing the centrifugate after recentrifuging with ethanol for 2 to 3 times to remove the ionic liquid remaining in the centrifugate;
• and
• (VII) resuspending the centrifugate obtained after washing in step (VI) with water, and then centrifuging and collecting a supernatant.