Double strand RNA-mediated RNA interference through feeding detrimental to larval Lymantria dispar (gypsy moth)
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsDawn E. Gundersen-Rindal, Saikat Kumar B. Ghosh
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Abstract
The present invention relates to the field of double-stranded RNA (dsRNA)-mediated gene silencing in insect species. More particularly, the present invention relates to genetic constructs designed for the expression of dsRNA corresponding to novel target genes in the insect pest Lymantria dispar, commonly known as the gypsy moth. The present invention also provides methodologies for introducing dsRNA into target insects to induce RNA interference. These constructs are particularly useful in RNAi-mediated control of the gypsy moth.
Claims21
A double-stranded ribonucleic acid (dsRNA) comprising a sense region with at least 99% or 100% sequence identity to SEQ ID NO:
- 2, and an antisense region comprising a second sequence complementary to the sense region.
A double-stranded ribonucleic acid (dsRNA) comprising a sense region comprising a sequence with at least 99% or 100% sequence identity to at least 21 consecutive nucleotides of SEQ ID NO:
- 2, and an antisense region comprising a second sequence complementary to the sense region.
A DNA molecule comprising a promoter functional in a host cell and a DNA encoding a dsRNA comprising a first region and a second region, wherein the first region comprises a sense region with at least 99% or 100% sequence identity to SEQ ID NO:
- 2, and a second region complementary to the first region.
Dependencies are inferred from the claim text.