Regulated switch for gene expression
AssigneeThe Johns Hopkins University
InventorsShigehito Ikushima, Jef Boeke
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Abstract
A system for controlling gene expression in yeast comprises a repressible gene expression plasmid that has a regulator binding sequence for camR and a target gene sequence. The system also includes a transcription enhancer expression plasmid; wherein said transcriptional activator protein binds to the regulator binding sequence in the absence of a transcriptional inhibitor. The system is used in a method for controlling expression of the target gene through the use of camphor. The target gene is expressed in the absence of camphor but unexpressed if camphor is added to a solution of cells containing the plasmids.
Claims15
A system comprising for controlling expression of a gene of interest in yeast comprising:
- (a) a first plasmid comprising (i) an operon for camphor repressor (camR);
- and (ii) a gene of interest downstream of the operon for camR;
- and
- (b) a second plasmid comprising (i) a nucleotide sequence encoding camR,
- wherein, in the absence of camphor, camR binds to the camR operon and drives expression of the gene of interest, and
- wherein, in the presence of camphor, camR does not bind to the camR operon and the gene of interest is not expressed.
Dependencies are inferred from the claim text.