Recombinant yeast expressing rubisco and phosphoribulokinase
AssigneeTechnische Universiteit Delft
InventorsAntonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Victor Gabriel Guadalupe Medina, Hendrik Wouter Wisselink
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Abstract
The invention relates to a recombinant yeast cell, in particular a transgenic yeast cell, functionally expressing one or more recombinant, in particular heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK). The invention further relates to the use of carbon dioxide as an electron acceptor in a recombinant chemotrophic micro-organism, in particular a eukaryotic micro-organism.
Claims19
A recombinant yeast cell comprising one or more recombinant heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK), wherein the nucleic acid sequence encoding the Rubisco comprises the sequence of SEQ ID NO:
- 1, and
- wherein the recombinant yeast cell further comprises nucleic acid sequences encoding one or more prokaryotic molecular chaperones selected from the group consisting of GroEL, GroES, a functional homologue of GroEL, and a functional homologue of GroES, wherein the respective GroEL and GroES homologues are functional as molecular chaperones.
One or more vectors for the expression of a heterologous polypeptide in a yeast cell, wherein:
- said vector or vectors comprise one or more recombinant-heterologous nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK);
- said encoded Rubisco exhibits carbon fixation activity;
- and
- the nucleic acid sequence encoding the Rubisco comprises the sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.