Recombinant Bacillus subtilis for producing acetylglucosamine and construction method thereof

A construction method of a recombinant Bacillus subtilis for producing acetylglucosamine, comprising:

• (1) constructing a deleting cassette of a glmS ribozyme encoding gene, wherein the deleting cassette includes an upstream homologous fragment, a resistance gene, a terminator sequence, a constitutive promoter sequence and a downstream homologous fragment in sequence;
• and
• (2) transforming the deleting cassette of the step (1) into Bacillus subtilis, to obtain the recombinant Bacillus subtilis,
• wherein the upstream homologous fragment is amplified by using an up-stream homologous fragment primer having a nucleic acid sequence as shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:9, SEQ ID NO:15, or SEQ ID NO:21,
• wherein the terminator is a trp terminator having a nucleic acid sequence as shown in SEQ ID NO:27, a ybc terminator having a nucleic acid sequence as shown in SEQ ID NO:29, or T7 terminator having a nucleic acid sequence as shown in SEQ ID NO:28,
• wherein the constitutive promoter is a P43 promoter or a PsrfA promoter,
• wherein the P43 promoter is amplified by using a P43 promoter primer having a nucleic acid sequence as shown in SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:24, or SEQ ID NO:25, and
• wherein the PsrfA promoter is amplified by using a PsrfA promoter primer having a nucleic acid sequence as shown in SEQ ID NO:18 or SEQ ID NO:19.