Double-stranded RNA constructs to control insect pests
AssigneesThe United States of America as represented by the Secretary of Agriculture, Bayer CropScience AG
InventorsRobert K. Vander Meer, Man Yeon Choi, Frederic Paul Schmitt, Cecile Denise Dorme, Angela Becker
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Abstract
Disclosed is a dsRNA construct that relates to a method to control insect pests of the genus Helicoverpa or Heliothis double-stranded RNA interference of the PBAN/Pyrokinin gene.
Claims13
A double-stranded ribonucleic acid (dsRNA) comprising a sense region comprising a sequence with at least 99% sequence identity to at least 21 consecutive nucleotides of SEQ ID NO:
- 7 or SEQ ID NO:
- 8, and an antisense region comprising a second sequence complementary to said sense region.
A DNA comprising a promoter functional in a host cell, and a DNA encoding a dsRNA comprising a first and a second region, wherein said first region comprises a sequence with at least 99% sequence identity to a portion of at least 21 consecutive nucleotides of SEQ ID NO:
- 7, or SEQ ID NO:
- 8, and wherein said second region is complementary to said first region.
A chimeric gene comprising the following operably linked DNA:
- (a) a plant-expressible promoter;
- (b) a DNA region which when transcribed yields a double-stranded RNA molecule targeting a PBAN/pyokinin gene of a Heliothis insect, said RNA molecule comprising a first and second RNA region wherein:
- (i) said first RNA region comprises a nucleotide sequence of at least 99% sequence identity to at least 21 consecutive nucleotides of SEQ ID NO:
- 7 or SEQ ID NO:
- 8;
- (ii) said second RNA region comprises a nucleotide sequence complementary to said first RNA region;
- and
- (iii) said first and second RNA region are capable of base-pairing to form a double-stranded RNA molecule;
- and
- (c) optionally, a 3′ end region comprising transcription termination and polyadenylation signals functioning in plant cells.
Dependencies are inferred from the claim text.