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US 10,093,922 B2Granted
Compositions and methods for creating altered and improved cells and organisms
OwnerHelge Zielerinventor
Life of this patent
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Abstract
The present invention provides compositions comprising randomized in-frame fusion polynucleotides and methods for introducing them into a host organism to identify desirable phenotypic changes that disrupt or alter existing genetic or biochemical mechanisms or pathways, thus creating novel characteristics of the transformed organism. Methods for using the compositions for increasing diversity within populations of organisms are also presented.
Claims16
A method of producing a polynucleotide library comprising:
- (a) creating a first polynucleotide collection of full-length open reading frames comprising a plurality of polynucleotides having non-homologous sequences identified and isolated from a sequenced genome of a single organism;
- (b) creating a second polynucleotide collection of full-length open reading frames comprising a plurality of polynucleotides having non-homologous sequences identified and isolated from a sequenced genome of a single organism;
- (c) optionally amplifying the isolated polynucleotide sequences of step (a) and/or step (b);
- and
- (d) joining in-frame in a random manner the collection of step (a) and the collection of step (b) to produce a library comprising a plurality of polynucleotides having sequences different from each other, each polynucleotide comprising a composite single open reading frame formed by at least two non-identical full-length open reading frames, at least one open reading frame originating from step (a) and at least one open reading frame originating from step (b), and encoding a single fusion polypeptide, wherein the at least one open reading frame originating from step (a) and the at least one open reading frame originating from step (b) are not homologous.
2 more elements
Dependencies are inferred from the claim text.