Scaffolded peptidic libraries and methods of making and screening the same

A library of distinct peptidic compounds, wherein the compounds of the library comprise one of following:

• a) a peptidic scaffold domain that is Chain C:LEM domain/emerin (SCF70) having a variable domain comprising 5 or more mutations at positions selected from 22, 23, 24, 25, 27, 29, 30, 33, 34, 36 and 37;
• b) a peptidic scaffold domain that is Transcription antitermination protein NusG (SCF42) having a variable domain comprising 5 or more mutations at positions selected from 16, 17, 18, 19, 22, 42, 43, 45, 47 and 55;
• c) a peptidic scaffold domain that is GYF domain of CD2bp2 (SCF47) having a variable domain comprising 5 or more mutations at positions selected from 42, 45, 46, 47, 48, 49, 51, 55, 57, 59, 60 and 61;
• d) a peptidic scaffold domain that is C-terminal domain of Ku (SCF28) having a variable domain comprising 5 or more mutations at positions selected from 1, 2, 4, 5, 8, 9, 12, 13, 14, 17, 19 and 22;
• e) a peptidic scaffold domain that is Fyn SH3 domain (Fynomers) (SCF63) having a variable domain comprising 5 or more mutations at positions selected from 13, 14, 15, 16, 30, 31, 32, 33, 34, 35, 36, 38 and 49;
• and
• f) a peptidic scaffold domain that is Chain C:
• GspC (SCF75) having a variable domain comprising 5 or more mutations at positions selected from 12, 13, 15, 16, 19, 23, 30, 32, 34, 51, 52, 67 and 68.