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US 10,093,920 B2Granted
Protein display
AssigneeAffinity Biosciences Pty Ltd
InventorsMatthew Beasley, Ben Kiefel
Life of this patent
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Abstract
The present invention relates to methods for screening a polypeptide for desired activity against a target molecule In particular, the present invention relates to methods for screening a polypeptide for desired activity against a target molecule by expressing the polypeptide in a bacterial cell and permeabilizing the cell.
Claims7
A method of screening a polypeptide for a desired activity against a target molecule, the method comprising:
- a) culturing a gram negative bacterial cell comprising a polynucleotide encoding the polypeptide such that the polypeptide is produced, folded and localized in the cytoplasm,
- b) permeabilising the bacterial cell with a non-ionic detergent or organic solvent, wherein the polypeptide and polynucleotide encoding the polypeptide are retained inside the permeabilised bacterial cell,
- c) contacting the permeabilised bacterial cell with the target molecule such that the target molecule diffuses into the permeabilised bacterial cell, and
- d) screening the polypeptide for the desired activity.
1 more element
Dependencies are inferred from the claim text.