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US 10,093,913 B2Granted
Methods for extracting and purifying non-denatured proteins
AssigneeImpossible Foods Inc.
InventorsAniket Kale, Ranjani Varadan, Simon Christopher Davis
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Abstract
Materials and methods for extracting and purifying proteins are provided. For example, the materials and methods provided herein can be used for extracting and purifying proteins that denature at low temperature.
Claims15
A method for purifying a soybean 7S protein, comprising:
- extracting a disintegrated soybean biomass to remove solids and generate a protein suspension;
- adding salt to the protein suspension;
- adjusting the pH of the protein suspension to a pH of 2 to 10;
- dialyzing the protein suspension against a polyethylene glycol (PEG) solution, or subjecting the protein suspension to ultrafiltration using a permeable membrane to separate the protein suspension on one side of the membrane from a permeate on the other side of the membrane, wherein PEG is present on the permeate side of the membrane;
- and
- subjecting the dialyzed or ultrafiltered protein solution to one or more concentrating or filtering steps to generate a product phase that contains the soybean 7S protein.
3 more elements
Dependencies are inferred from the claim text.