Nitrile hydratase
AssigneeMitsubishi Chemical Corporation
InventorsAya Kitahara, Takanori Akiyama, Miki Wakamatsu, Fumiaki Watanabe
Life of this patent
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Abstract
Provided is a novel improved nitrile hydratase with improved resistance to amide compounds under high temperatures. Specifically provided is a nitrile hydratase having at least one amino acid mutation selected from (a) to (d) below, in the amino acid sequence expressed in SEQ ID NO:50 (X1 to X27 represent independent arbitrarily-defined amino acid residuals). (a) X1 is valine or glycine (b) X9 is valine or threonine (c) X23 is an amino acid selected from a group consisting of isoleucine, leucine, methionine and threonine, (d) X24 is leucine.
Claims13
An isolated modified nitrile hydratase comprising an α subunit, wherein the α subunit comprises at least one amino acid sequence selected from the group consisting of SEQ ID NO:
- 46, 47, 48, and 49,
- wherein:
- (a) SEQ ID NO:
- 46:
- X1X2X3X4X5X6RX7KAX8E,
- wherein
- X1 is an amino acid other than tyrosine;
- X2, X3, X5, X6, X7 and X8 each independently are any amino acid residue;
- and X4 is tyrosine, and
- SEQ ID NO:
- 46 corresponds to amino acids in positions from 8 to 19 of SEQ ID NO:50;
- (b) SEQ ID NO:
- 47:
- X9X10X11 X12NX13X14X15X16X17X18X19X20X21CX22LC,
- wherein
- X9 is an amino acid other than serine;
- X10, X11, X12, X13, X14, X15, X16, X17, X19, X20, X21 and X22 each independently are any amino acid residue;
- and X18 is histidine, and
- SEQ ID NO:
- 47 corresponds to amino acids in positions from 88 to 105 of SEQ ID NO:50;
- (c) SEQ ID NO:
- 48:
- X23WDSX25X26EX27RX28X29V,
- wherein
- X23 indicates an amino acid other than valine;
- X25, X27, X28 and X29 each independently are any amino acid residue;
- and X26 is serine;
- and
- SEQ ID NO:
- 48 corresponds to amino acids in positions from 153 to 165 of SEQ ID NO:50;
- (d) SEQ ID NO:
- 49:
- VX24DSX25X26EX27RX28X29V,
- wherein
- X24 is an amino acid other than tryptophan;
- X25, X27, X28 and X29 each independently are any amino acid residue;
- and X26 is serine;
- and
- SEQ ID NO:
- 49 corresponds to amino acids in positions from 153 to 165 of SEQ ID NO:50.
Dependencies are inferred from the claim text.