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US 10,093,908 B2Granted
Recombinase polymerase amplification
AssigneeAlere San Diego, Inc.
InventorsOlaf Piepenburg, Niall A. Armes, Mathew James David Parker
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Abstract
The present invention features novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also features different recombinase ‘systems’ having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed.
Claims21
A composition comprising:
- (a) a T6 UvsX H66S protein,
- (b) a gp32 protein, and
- (c) a polymerase,
- wherein the gp32 protein is derived from a myoviridae phage.
1 more element
A composition comprising:
- (a) a T6 UvsX H66S protein,
- (b) a gp32 protein,
- (c) a polymerase,
- (d) at least one nucleic acid primer, and
- (e) a target nucleic acid,
- wherein the gp32 protein is derived from a myoviridae phage.
3 more elements
Dependencies are inferred from the claim text.