US 10,093,907 B2 · Hetero-transglycosylase and uses thereof

Abstract

The present invention relates to a hetero-transglycosylase protein having cellulose:xyloglucan endotransglucosylase (CXE) activity in addition to mixed-linkage beta-glucan:xyloglucan endotransglucosylase (MXE) activity. The protein may comprise the amino acid sequence of any one of SEQ ID NOs: 2, 6 and 8 or a functional fragment thereof; or an amino acid sequence having at least 60% sequence identity to any one of SEQ ID NO: 2, 6 and 8, or to SEQ ID NO: 2 from amino acid 22 to 280, to SEQ ID NO: 6 from amino acid 26 to 283, or to SEQ ID NO: 8 from amino acid 29 to 287. The invention furthermore relates to an isolated nucleic acid encoding the protein described herein, a chimeric gene comprising, inter alia, the nucleic acid described herein, a vector comprising said chimeric gene, a host cell comprising said vector or said chimeric gene and an according transgenic plant. Further disclosed herein in are a method of producing a transgenic plant and a method of improving properties of cellulosic material.

Claims15

Dependent claims

A chimeric gene comprising the following operably linked elements:

a. a heterologous promoter;
b. the nucleic acid selected from the group consisting of:
i. a nucleic acid sequence encoding a protein having cellulose:xyloglucan endotransglucosylase activity comprising
1. the amino acid sequence of any one of SEQ ID NOs:
2, 6 and 8;
or
2. an amino acid sequence having at least 95% sequence identity to the sequence of any one of SEQ ID NOs:
2, 6 and 8, or
3. an amino acid sequence having at least 95% sequence identity to the sequence of SEQ ID NO:
2 from amino acid 22 to 280, or to the sequence of SEQ ID NO:
6 from amino acid 26 to 283, or to the sequence of SEQ ID NO:
8 from amino acid 29 to 287,
ii. a nucleic acid sequence having the sequence of any one of SEQ ID NOs:
1, 5 and 7;
iii. a nucleic acid sequence having the sequence of SEQ ID NO:
1 from nucleotide 64 to 840, or the sequence of SEQ ID NO:
5 from nucleotide 76 to 849, or the sequence of SEQ ID NO:
7 from nucleotide 85 to 861
and, optionally,
c. a transcription termination and polyadenylation region.

17 more elements

Dependencies are inferred from the claim text.

Hetero-transglycosylase and uses thereof

Life of this patent

Hetero-transglycosylase and uses thereof

Life of this patent

Abstract

Claims15