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US 10,093,903 B2Granted
Production of virus-receptive pluripotent stem cell (PSC)-derived hepatocytes
AssigneeFUJIFILM Cellular Dynamics, Inc.
InventorsChristian Kannemeier, Elisabeth Enghofer, Lisa Harms
Life of this patent
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Abstract
The present disclosure provides methods for maturing hepatocytes comprising culturing with cyclic adenosine monophosphate and a Janus kinase inhibitor. There is also provided a method for screening inhibitors of hepatitis B virus infection and/or replication.
Claims24
An in vitro method of producing virus-receptive pluripotent stem cell-derived hepatocytes comprising:
- (a) obtaining pluripotent stem cell (PSC)-derived hepatocytes;
- and
- (b) culturing the PSC-derived hepatocytes in media comprising cyclic adenosine monophosphate (cAMP) and a Janus kinase inhibitor (JAKi), thereby producing virus receptive hepatocytes.
Dependencies are inferred from the claim text.