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US 10,093,902 B2Granted
Human adipose tissue white and ‘brown-on-white’ progenitors for reconstructive and metabolic therapies
AssigneeUniversity of Massachusetts
InventorSilvia Corvera
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Abstract
Methods for preparation of prospectively identified human adipose stem cells enriched populations thereof, e.g., for therapy.
Claims7
A method of making a population of brite adipose cells from Human Adipose Capillary Progenitor Cells (HACAPS), the method comprising:
- providing primary adipose cells or tissue from a subject, and
- (i) culturing the primary cells or tissue in the presence of pro-angiogenic factors sufficient to induce growth of a population of cells comprising capillary cells, wherein the pro-angiogenic factors comprise human recombinant FGF-2 in combination with one or more of VEGF, IGF1, and EGF, and then
- (ii) isolating single cells from the population of cells to form a population of cells enriched for HACAPS, and
- (iii) maintaining the HACAPS in culture in the presence of adenylate cyclase activators or adrenergic agonists to induce differentiation of the HACAPS into brite adipose cells.
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Dependencies are inferred from the claim text.