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US 10,093,898 B2Granted
Purification of functional human astrocytes
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsYe Zhang, Steven Sloan, Ben A. Barres
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Abstract
Compositions and methods are provided for the purification of astrocytes from biological samples or from in vitro cultures. An advantage of the methods of the invention is the ability to isolate astrocytes in a quiescent state, which allows analysis of the cells in a more natural state.
Claims10
A method for isolation of functional, quiescent human astrocytes, the method comprising:
- contacting a complex population of human brain cells comprising astrocytes with at least one negative selection antibody or lectin that selectively binds a non-astrocyte lineage selected from anti-CD45;
- anti-GalC, anti-O4, anti-CD90 and Banderiaea simplicifolia lectin 1 (BSL-1) and collecting the eluate containing non-selected cells;
- and
- contacting the selected cell population with an antibody that specifically binds to HepaCAM, and collecting cells bound to the antibody;
- to generate a purified population of astrocytes.
2 more elements
Dependencies are inferred from the claim text.