Generation of neuronal cells from pluripotent stem cells
AssigneeUniversite Libre de Bruxelles
InventorsPierre Vanderhaeghen, Nicolas Gaspard, Gilles Naeije, Jelle Van Den Ameele, Fabienne Devreker, Yvon Englert
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Abstract
The invention relates to in vitro methods for differentiating mammalian pluripotent stem cells into cells displaying a neuronal phenotype, more particularly into cortical-type neurons including inter alia pyramidal neurons and cortical inhibitory interneurons. The invention further encompasses so-obtained neuronal cells and cell population, compositions comprising such, and further uses of said neuronal cells and cell population.
Claims17
A method for differentiating mammalian induced pluripotent stem (iPS) cells into neural progenitors of dorsal forebrain identity comprising the steps of:
- a) plating undifferentiated mammalian iPS cells onto a substrate which allows adherence of cells thereto;
- and
- b) culturing the mammalian iPS cells of a) which have adhered to said substrate in a medium permissive to differentiation of the mammalian iPS cells comprising exposing the cells to an antagonist of the sonic hedgehog (SHH) signaling pathway during at least part of the culturing step, whereby neural progenitors of dorsal forebrain activity are obtained.
A method for differentiating mammalian iPS cells into neural progenitors of ventral forebrain identity comprising the steps of:
- a) plating undifferentiated mammalian iPS cells onto a substrate which allows adherence of cells thereto;
- and
- b) culturing the mammalian iPS cells of a) which have adhered to said substrate in a medium permissive to differentiation of the mammalian iPS cells comprising exposing the cells to an agonist of the SHH signaling pathway and to an antagonist of the Wnt signaling pathway during at least part of said culturing step, whereby neural progenitors of ventral forebrain identity are obtained.
A method for differentiating mammalian iPS cells into cortical pyramidal neuron like cells comprising the steps of:
- a) Plating undifferentiated mammalian iPS cells onto a substrate which allows adherence of cells thereto;
- and
- b) culturing the mammalian iPS cells of a) which have adhered to said substrate in a medium permissive to differentiation of the mammalian iPS cells comprising exposing the cells to an antagonist of the sonic hedgehog (SHH) signaling pathway during at least part of the culturing step, whereby cortical pyramidal neuron like cells are obtained.
A method for differentiating mammalian iPS cells into cortical or striatal inhibitory interneuron or striatal projection neuron like cells comprising the steps of:
- a) plating undifferentiated mammalian iPS cells onto a substrate which allows adherence of cells thereto;
- and
- b) culturing the mammalian iPS cells of a) which have adhered to said substrate in a medium permissive to differentiation of the mammalian iPS cells comprising exposing the cells to an agonist of the SHH signaling pathway and to an antagonist of the Wnt signaling pathway during at least part of said culturing step, whereby cortical or striatal inhibitory interneuron or striatal projection neuron like cells are obtained.
Dependencies are inferred from the claim text.