Cell culturing system for cultivating adherent cells and liquid supply interface comprising a cell culture container

A liquid supply interface for a cell culture system for supplying cell cultures found in different cell culture containers with a nutrient medium, wherein the liquid supply interface comprises:

• a housing defining a flow area;
• a first connection formation for the liquid-transferring connection of a first fluid line to the housing;
• a second connection formation formed separately from the first for the liquid-transferring connection of a second fluid line with the housing;
• a third connection formation formed separately from the first two for the liquid-transferring connection of the housing with a third fluid line;
• a coupling formation formed separately from the connection formations, which is formed for the producible and detachable liquid-transferring coupling contact according to the operation, with a corresponding counter-coupling formation of a cell culture container;
• a first liquid flow path, which extends between the flow area and the first connection formation for introducing a first liquid from the outside into the flow area;
• a second liquid flow path, which extends between the flow area and the second connection formation for introducing a second liquid different from the first from the outside into the flow area;
• a third liquid flow path, which extends between the flow area and the third connection formation for removing a liquid from the flow area;
• and
• a coupling flow path, which extends between the flow area and the coupling formation in order to remove a liquid from the flow area and/or to introduce it to said flow area via the coupling formation,
• wherein the first, the second, and the third liquid flow paths each have a valve configuration, which is completely surrounded with the exception of the respective liquid flow path by the housing, incorporated in it, without a continuous signal and/or power-transferring physical connection surrounded by the valve configuration up to the outside of the housing;
• wherein a control configuration with a signaling means generating an electric and/or magnetic and/or electromagnetic field is assigned to each valve configuration, the field of which acts upon a valve body of the valve configuration without contact, wherein each valve configuration can be switched off via the field, acting upon its valve body, between a blocked position, in which the valve configuration interrupts a liquid flow in the liquid flow path in which it is arranged, and an outlet position in which the valve configuration enables a liquid flow;
• wherein the coupling formation is located in a flow path between the valve configuration of the second connection formation and the valve configuration of the third connection formation;
• wherein at least a part of the valve configurations, in a passage flow direction along the liquid flow path, in which it is arranged, but not in the opposite flow direction, is adjustable from the blocked position to the outlet position by a predetermined liquid pressure difference;
• wherein the passage flow directions of the first and second liquid flow paths are each directed into the flow area.